Sterilization composition
A composition and crop technology, applied in the field of pesticides, can solve problems such as ineffective control of disease development, environmental pollution, and increased costs
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example 1
[0013] Bioassay example 1: Indoor virulence determination of pyraclostrobin and fluopyram for cucumber downy mildew
[0014] Test object: Cucumber downy mildew (Pseudoperonospora cubensis), collected from the field.
[0015] Test method: The test adopts the leaf disc method, weighs a certain amount of pyraclostrobin and fluopyram technical, dissolved in DMF and prepared into mother liquor, diluted with 0.1% Tween-80 surfactant sterilized water, in On the basis of preliminary experiments, 7 suitable series of mass concentrations were prepared. The two medicinal liquids of pyraclostrobin and fluopyram are mixed according to different mass ratios (50:1, 40:1, 30:1, 20:1, 10:1, 1:1, 1:10, 1: 20, 1:30, 1:40, 1:50) to obtain mixtures of different proportions, and set up a treatment containing only solvents and surfactants without active ingredients as a blank control. Collect fresh cucumber downy mildew diseased leaves in the field, wash the sporangia on the back of the leaves with dei...
example 2
[0028] Bioassay example 2: Indoor virulence determination of pyraclostrobin and fluopyram in a pair of Phytophthora infestans
[0029] Test object: Phytophthora infestans, collected from the field, and separated and purified indoors.
[0030] Test method: The test adopts the leaf disc method. Weigh a certain amount of pyraclostrobin and fluopyram, dissolve it in DMF and prepare the mother liquor, dilute with 0.1% Tween-80 surfactant sterilized water, and prepare 7 on the basis of preliminary experiments Suitable series mass concentration. The two medicinal liquids of pyraclostrobin and fluopyram are mixed according to different mass ratios (50:1, 40:1, 30:1, 20:1, 10:1, 1:1, 1:10, 1: 20, 1:30, 1:40, 1:50) to obtain mixed solutions of different proportions, and set up a treatment containing only solvents and surfactants without active ingredients as a blank control. Wash the cultured sporangia of Phytophthora infestans with sterile water and filter with double gauze to prepare a ...
example 3
[0035] Bioassay example 3: Indoor virulence determination of pyraclostrobin and fluopyram in pairs of Phytophthora capsici
[0036] Test object: Phytophthora capsici Leonian, collected from the field, and separated and purified indoors.
[0037] Test method: Refer to "The People's Republic of China Agricultural Industry Standard NY / T1156.2-2006", using the mycelial growth rate method. Under aseptic conditions, the cultivated capsicum phytophthora spp. was used to take the fungus cake along the edge of the hypha with a sterile punch with a diameter of 5 mm, and the fungus cake was inoculated in the center of the medicated plate with the inoculation needle, with the hyphae facing down. Place in a light incubator at 25°C. According to the survey results of the growth of the control hyphae, the cross method was used to measure the colony diameter of each treatment, and the percentage of inhibition (%) of different agents on the growth of the hyphae was calculated according to the foll...
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