Method for preparing high-purity sulforaphane

Abstract

The invention provides a method for preparing high-purity sulforaphane and in particular relates to a method for extracting the sulforaphane from radish seeds. The method is used for preparing products having the purity of more than 98% from air-dried radish seeds as the raw material and through the steps of cracking, degreasing, hydrolysis, initial separation by use of resin, reverse osmosis concentration, preparation separation and freeze-drying. The method is simple and convenient to operate, simple in equipment, mild in reaction conditions, energy-saving, low in production cost, and convenient to popularize and utilize. The used methanol waste liquid is recovered, the used resin is regenerated and impurities generated can be recycled, and therefore, the production is safe, green and pollution-free, and the production method is capable of saving energy and reducing emission. The sulforaphane product prepared by the method can be widely applied to the industries such as medicines and health care products.

Description

1. Technical field [0001] The invention belongs to the technical field of separation and preparation of sulforaphane, in particular to a method for extracting sulforaphane from radish seeds. 2. Background technology [0002] Glucosinolate is a kind of glucosinolate in cruciferous vegetables. Under the catalysis of glucosinosidase or acid, glucosinolate is hydrolyzed to generate isothiocyanates such as sulforaphane and glucose. Scientific research has found that the chemical name of sulforaphane is 1-isothiocyanate-4-methanesulfonyl butane or 1-isothiocyanate-4-methanesulfonyl-(2-ene)butane, which is by far the One of the strongest anti-cancer ingredients found in vegetables, it can induce the body to produce type II detoxification enzymes, which can prevent carcinogens from destroying genetic factors in healthy cells, so 1-isosulfur is isolated and purified from cruciferous vegetables Cyanate-4-methanesulfonylbutane or 1-isothiocyanate-4-methanesulfonyl-(2-ene)butane has im...

AI Technical Summary

Problems solved by technology

The main problems in this method are: 1. The seeds are not degreased. Due to the blocking of oil, the sinaprin and glucuronidase in the broccoli seeds are difficult to effectively contact, resulting in a low production rate of sulforaphane; 2. Immobilization of the extracted myrosinase, the free enzyme has a short half-life and is easily inactivated; 3. The organic solvent used - acetone has a strong ability to denature the enzyme protein, resulting in a large amount of denaturation and inactivation of the myrosinase

The main problems in this method are: 1. the optimal pH value required for glucosinosidase is not adjusted during hydrolysis, resulting in low activity of glucosinosidase and low sulforaphane yield; 2. using organic solvents Ethyl acetate and acetonitrile, both solvents are volatile, have heavy odor, are toxic, and cause serious air pollution; therefore, the cost of sulforaphane produced by this method is also high and lacks competitiveness