Strong strawberry seedling rooting culture method
A technology of rooting and cultivation methods for strong seedlings, applied in the field of botany, can solve problems such as early fruiting, high labor intensity, low yield and diseases, and achieve the effects of strong seedling growth, increased yield and quality, and enhanced economic value
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[0020] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,
[0021] And the components of the macronutrient elements and their corresponding concentrations are: the components of the macronutrient elements and their corresponding concentrations are: potassium nitrate 1500mg / L; ammonium sulfate 1560mg / L; calcium chloride dihydrate 320mg / L ; Potassium dihydrogen phosphate anhydrous 100mg / L; Disodium edetate 28.6mg / L; Ferrous sulfate heptahydrate 20.5mg / L,
[0022] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 19.5mg / L; zinc sulfate 9.0mg / L; boric acid 7.5mg / L; potassium iodide 1.0mg / L; sodium molybdate 0.5mg / L L; cobalt chloride 0.05mg / L,
[0023] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 15.0 mg / L; nicotinic acid 1.5 mg / L; pyridoxine hydrochloride 1.5 mg / L; inositol 60.0 mg / L....
Embodiment 1
[0026] The strong seedling rooting culture method of strawberry of the present invention is specifically:
[0027] (1) Seed selection and disinfection: choose the strawberry stem section with the stem tip growth point, rinse it with running water first, then soak it in 70% ethanol solution for 40s, then soak it in sterile water for 5min, rinse it 3 times with sterile water, Then choose the shoot tip of 0.5-0.8mm and inoculate it in the strong seedling rooting medium, and the strong seedling rooting medium includes 1 / 2 improved MS+0.2mgL -1 IBA+0.1mgL -1 NAA+0.5mg indolebutyric acid+1.0gL -1 Activated carbon + 100ml / L banana juice + 4.0gL -1 Agar+20gL -1 Sucrose, and the pH value is 5.8;
[0028] (2) rooting culture of strong seedlings:
[0029] During rooting culture, keep the temperature at 25-28°C, the light time is 12-15h per day, and the light intensity is 2800-3000lx, and cultivate for 3 weeks. When the root length of the rooted plant reaches 0.4-0.6cm, transplant it...
Embodiment 2
[0033] The strong seedling rooting culture method of strawberry of the present invention is specifically:
[0034] (1) Seed selection and disinfection: choose the strawberry stem section with the stem tip growth point, rinse it with running water first, then soak it in 70% ethanol solution for 50s with a concentration, then soak it in sterile water for 6 minutes, rinse it 5 times with sterile water, Then choose the shoot tip of 0.5-0.8mm and inoculate it in the strong seedling rooting medium, and the strong seedling rooting medium includes 1 / 2 improved MS+0.3mgL -1 IBA+0.3mgL -1 NAA+0.3mg indolebutyric acid+2.0gL -1 Activated carbon + 100ml / L banana juice + 5.0gL -1 Agar+20gL -1 Sucrose, and the pH value is 5.8;
[0035] (2) rooting culture of strong seedlings:
[0036] During rooting culture, keep the temperature at 25-28°C, the light time is 12-15h per day, and the light intensity is 2800-3000lx, and cultivate for 5 weeks. When the root length of the rooted plant reache...
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