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Camellia nitidissima tea active site, preparation method and application thereof

A technology of golden camellia tea and active parts, which is applied in the field of active parts of traditional Chinese medicine

Inactive Publication Date: 2014-09-10
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on its hypoglycemia and inhibition of a-glucosidase activity

Method used

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  • Camellia nitidissima tea active site, preparation method and application thereof

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Effect test

preparation example Construction

[0018] One embodiment of the present application provides a method for preparing the active fraction of Camellia japonica, which includes the following steps:

[0019] A. Infiltration: take the coarse powder of golden camellia tea, soak overnight with 5 times (V / W) alcohol solution of 30% or more concentration;

[0020] B. Extraction: use the alcoholic solution of the same concentration as in step A to heat and reflux the golden camellia tea coarse powder in A step to extract 2 times or more than 2 times, and the alcohol solution consumption is 10~40 times of golden camellia coarse powder (V / W), each extraction time is 1 to 4 hours, filtered, combined extracts, recovered solvent, to obtain a concentrated paste;

[0021] C. Extraction: Suspend the concentrated paste with water, extract with petroleum ether, ethyl acetate, n-butanol successively, take the extract after ethyl acetate extraction, and reclaim the solvent;

[0022] D. Drying: Freeze-dry the ethyl acetate extract o...

Embodiment 1

[0034] The preparation of embodiment 1 golden camellia active fraction

[0035] Golden camellia tea coarse powder 1000g, soaked 5 times in 70% ethanol overnight, heated and refluxed for 3 times, 3 hours for the first time, 2.5 hours for the second and third times respectively, combined the extracts, recovered the solvent to obtain a concentrated paste, and then used petroleum ether , ethyl acetate, and n-butanol extraction, take the ethyl acetate extract, recover the solvent, and freeze-dry the obtained ethyl acetate to obtain the product.

Embodiment 2

[0036] Embodiment 2 pharmacological activity test

[0037] 1) Reagent

[0038] a-glucosidase (a-glucosidase, EC3.2.1.20), 4-nitrophenyl-a-D-glucopyranoside (4-Ntrophenyl-a-D-glucopyranoside, PNPG, 026K1516), acarbose (Acarbose, Lot16869, positive control) and DMSO were purchased from Sigma, and bovine serum albumin (5 components) (BSA) was purchased from Solarbio Co., Ltd.

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Abstract

The invention discloses a Camellia nitidissima tea active site, a preparation method and application thereof. The preparation method includes: A. infiltration: taking coarse Camellia nitidissima tea powder, and soaking the powder overnight with a quintuple (V / W) alcoholic solution with a concentration of 30% or above; B. extraction: employing an alcoholic solution with a same concentration to that of step A to conduct heating reflux extraction on the coarse Camellia nitidissima tea powder in step A twice or more, with the dosage of the alcoholic solution being 10-40 times (V / W) that of the coarse Camellia nitidissima tea powder, controlling the extraction time at 1-4h each time, performing filtration, merging the extracted liquid, and recovering the solvent so as to obtain a concentrated paste; C. extraction: suspending the concentrated paste with water, carrying out extraction with petroleum ether, ethyl acetate and n-butyl alcohol in order, taking the extracted liquid of ethyl acetate, and recovering the solvent; and D. drying: freeze drying the ethyl acetate extract obtained in step C, thus obtaining the Camellia nitidissima tea active site.

Description

technical field [0001] The present invention relates to active part of traditional Chinese medicine, its preparation method and application. Background technique [0002] α-glucosidase plays an important role in the absorption process of food. Food must be combined with this enzyme to be digested and absorbed to increase blood sugar. α-Glucosidase inhibitors are a new type of oral hypoglycemic drugs widely used at present. Acarbose, voglibose and miglitol are commonly used in clinical practice. Inhibit the a-glucosidase of the brush border of the mesentery, delay the decomposition of polysaccharides (such as starch, oligosaccharides, etc.) into glucose by a-glucosidase, thereby slowing down the absorption rate of glucose and reducing postprandial blood sugar. Acarbose mainly inhibits a-amylase and acts on the digestion process of macromolecular polysaccharides; voglibose and miglitol selectively inhibit disaccharide hydrolase (maltase, sucrase) and decompose disaccharides ...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61K8/97A61P3/10
Inventor 冯洁赖茂祥
Owner GUANGXI MEDICAL UNIVERSITY
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