Camellia nitidissima tea active site, preparation method and application thereof
A technology of golden flower tea leaves and active parts, which is applied in the directions of food preparation, application, pharmaceutical formulation, etc.
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[0018] One embodiment of the present application provides a method for preparing the active fraction of Camellia japonica, which includes the following steps:
[0019] A. Infiltration: take the coarse powder of golden camellia tea, soak overnight with 5 times (V / W) alcohol solution of 30% or more concentration;
[0020] B. Extraction: use the alcoholic solution of the same concentration as in step A to heat and reflux the golden camellia tea coarse powder in A step to extract 2 times or more than 2 times, and the alcohol solution consumption is 10~40 times of golden camellia coarse powder (V / W), each extraction time is 1 to 4 hours, filter, combine the extracts, reclaim the solvent to obtain a crude extract, filter, and take the filtrate to obtain a medicinal solution with a specific gravity of 1.05 to 1.25;
[0021] C. macroporous adsorption resin treatment: the concentrated paste is suspended in water, separated by macroporous resin column chromatography, and eluted with 10...
Embodiment 1
[0034] The preparation of the active part of embodiment 1 golden camellia
[0035] Golden camellia tea coarse powder 1000g, soaked 5 times in 70% ethanol overnight, heated and refluxed for 3 times, 3 hours for the first time, 2.5 hours for the second and third times respectively, combined the extracts, recovered the solvent, obtained the crude extract, filtered, and took The filtrate, the specific gravity of the medicinal solution is 1.05 to 1.25, and the obtained filtrate is separated by D101 macroporous resin column chromatography, and eluted with 10%, 30%, 80%, and 95% ethanol water of 10 column volumes successively, and the eluents are collected respectively. The 80% ethanol water eluted part was used to recover the solvent, freeze-dried to obtain the 80% ethanol part.
Embodiment 2
[0036] Embodiment 2 pharmacological activity test
[0037] 1) Reagent
[0038] a-glucosidase (a-glucosidase, EC3.2.1.20), 4-nitrophenyl-a-D-glucopyranoside (4-Ntrophenyl-a-D-glucopyranoside, PNPG, 026K1516), acarbose (Acarbose, Lot16869 , positive control) and DMSO were purchased from Sigma, and bovine serum albumin (5 components) (BSA) was purchased from Solarbio Co., Ltd.
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