Method for extracting rice bran protein from defatted rice bran by bead grinding breaking desmoenzyme method

Abstract

A method for extracting rice bran protein from defatted rice bran by bead grinding breaking desmoenzyme method comprises the following steps: (1) crushing defatted rice bran meal, adding water for soaking; (2) grinding the obtained material liquid; (3) adding an acetic acid-sodium acetate buffer, adjusting pH to 4-4.8, adding cellulase, stirring in water bath, then inactivating enzyme, and then cooling; (4) adding alpha-amylase, stirring in the water bath, regulating the pH, precipitating rice bran protein, removing supernatant to obtain crude rice bran protein; (5) resuspending, filtering, performing vacuum spray drying, and collecting rice bran protein powder. By adopting the method, the extraction efficiency of the rice bran protein is increased significantly, the purity is higher, the extraction condition is mild, the nature of the product is good, and rice bran residue after the extraction can also be used as animal feeds.

Description

technical field [0001] The invention relates to a method for extracting rice bran protein, in particular to a method for extracting rice bran protein from defatted rice bran by bead milling and crushing combined with an enzyme method. Background technique [0002] Currently, the main methods of extracting rice bran protein from rice bran include alkali-dissolved acid extraction and enzymatic hydrolysis extraction. In the former extraction process, a large amount of lye and acid are added, and the reaction is violent. Under high alkaline conditions, the protein in rice bran is destroyed. Seriously, the quality of rice bran protein is affected, and the denatured protein does not have its original activity; and it may also generate toxic substances, which are still difficult to remove even through charcoal adsorption and other methods, so the potential toxicity of its products determines that it is not suitable for use in Food and drug deep processing field. [0003] The enzym...

AI Technical Summary

Problems solved by technology

Since each rice bran powder is composed of rice bran cells, it is difficult for the enzyme agent to fully contact the rice bran cell wall, and the protein structure network in the cell wall can only act on the surface of the particle by simply using cellulase and phytase It is difficult to crack all the cell walls. If you want to improve the extraction efficiency, you need to use protease or increase the amount of enzyme solution or even prolong the reaction time. The former will degrade the rice bran protein, while the latter will greatly increase the production cost. Suitable for large-scale industrial production

[0004] In addition, there are some extraction methods that use high-temperature cooking or high-pressure crushing. Although the yield is higher, the rice bran protein is severely denatured, and even broken into short peptides, losing its biological function.