Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Regulation of receptor expression through delivery of artificial transcription factors

A technology of artificial transcription factors and receptors, applied in animal/human peptides, antibody mimics/scaffolds, peptide sources, etc.

Inactive Publication Date: 2014-08-20
ALIOPHTHA
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While ETRA is located in the smooth muscle cells that form the walls of blood vessels and promotes vasoconstriction, ETRB is mainly expressed in endothelial cells and causes vasodilation by promoting the release of nitric oxide, thereby causing smooth muscle relaxation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Regulation of receptor expression through delivery of artificial transcription factors
  • Regulation of receptor expression through delivery of artificial transcription factors
  • Regulation of receptor expression through delivery of artificial transcription factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0183] Cloning of DNA plasmids

[0184] For all cloning steps, restriction endonucleases and T4 DNA ligase were purchased from New England Biolabs. Shrimp alkaline phosphatase (SAP) was from Promega. Hi-Fi Platinum Pfx DNA polymerase (Invitrogen) was used in all standard PCR reactions. DNA fragments and plasmids were isolated using NucleoSpin Extract II kit, NucleoSpin Plasmid kit or NucleoBond Xtra Midi Plus kit (Macherey-Nagel) according to manufacturer's instructions. Oligonucleotides were purchased from Sigma-Aldrich. All relevant DNA sequences of newly generated plasmids were verified by sequencing (Microsynth).

[0185] Design and cloning of two hexameric zinc finger proteins (ZFP-855A and ZFP+74A)

[0186] To generate an artificial transcription factor that regulates ETRA expression, a fusion protein consisting of TAT-KRAB-ZFP was designed and the corresponding, codon-optimized DNA sequence was obtained by gene synthesis. For the ZFP portion of the fusion pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an artificial transcription factor comprising a polydactyl zinc finger protein targeting specifically a receptor gene promoter fused to an inhibitory or activatory protein domain, a nuclear localization sequence, and a protein transduction domain. In particular examples these receptor gene promoters regulate the expression of the endothelin receptor A, the endothelin receptor B, the Toll-like receptor 4 or the high-affinity IgE receptor. Artificial transcription factors directed to the endothelin A or B receptors are useful in the treatment of diseases modulated by endothelin, such as cardiovascular diseases, and, in particular, eye diseases, e.g. retinal vein occlusion, retinal artery occlusion, macular edema, optic neuropathy, central serous chorioretinopathy, retinitis pigmentosa, Leber's hereditary optic neuropathy, and the like. Artificial transcription factors directed to the Toll-like receptor 4 or the IgE receptor are useful for the treatment of autoimmune disorders, and the like, and allergic disorders, respectively.

Description

field of invention [0001] The present invention relates to artificial transcription factors comprising multi-finger zinc finger proteins specifically targeting receptor gene promoters fused to inhibitory or activation domains, nuclear localization sequences and protein transduction domains, and their role in the treatment of specific Use in diseases modulated by binding of effectors to such receptors. Background technique [0002] proposed that artificial transcription factors (ATFs) are useful tools for regulating gene expression (Sera T., 2009, Adv Drug Deliv Rev 61, 513-526). Many naturally occurring transcription factors, which affect expression by repressing or activating gene transcription, have complex specific domains that recognize a certain DNA sequence. This makes them unattractive targets for manipulation if the skilled person intends to modify their specificity and target gene(s). However, a certain class of transcription factors contains several so-called z...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/62C07K14/00
CPCC07K14/4702C07K2319/09C07K2319/81C07K2319/10C07K2319/71C07K14/47A61P11/00A61P13/12A61P25/00A61P27/02A61P43/00A61P9/02A61P9/10A61P9/12
Inventor J.弗拉梅A.纽茨纳A.赫克斯利
Owner ALIOPHTHA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com