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A pear tree branch degrading fungus and its agent

A fungal inoculant and a technology for pear trees, which are applied in the field of degrading fungi on pear branches and their inoculants, can solve problems such as inability to return to the field, air pollution, pests and diseases, and achieve sustainable agricultural development, broad application prospects, protection of The effect of the environment

Active Publication Date: 2016-06-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, pear tree branches contain a large amount of lignin and cellulose, so they are not easy to rot and cannot be returned to the field in situ.
Except for some branches used in the cultivation of edible fungi, most of the branches of pear trees are randomly stacked on the roadside or burned on the spot. Burning will cause serious air pollution. waste of

Method used

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  • A pear tree branch degrading fungus and its agent
  • A pear tree branch degrading fungus and its agent
  • A pear tree branch degrading fungus and its agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and identification of pear branch degrading fungus D10

[0029] 1) Screening of degrading fungi from pear tree branches: The samples of degrading bacteria were collected from rotten pear tree branches piled up all year round in Xinji, Hebei. Enriched by solid-state enrichment medium with pear tree branches as the only carbon source for 30 days (solid-state enrichment medium is: 20 g of pear tree branch powder passed through a 20-mesh sieve after drying and crushing, and 20 g of ultrapure water is evenly added) , select well-growing and fast-growing colonies, and inoculate them on a new solid-state enrichment medium to continue culturing for the second generation to obtain strains with high-efficiency degradation effects on pear tree branches. Inoculate the well-growth bacterium colonies on the solid-state enrichment medium into the guaiacol selection medium (guaiacol selection medium is: guaiacol 1g, KNO 3 2g, MgSO 4 0.5g, KH 2 PO 4 1g, NaCl1g, N...

Embodiment 2

[0037] The preparation of embodiment 2 pear branch degrading bacteria D10 inoculum

[0038] Inoculate strain D10 on pear branch powder medium, culture statically at 30°C for 7 days, add 30mL sterile water and shake on a shaker for 30min, filter the culture with gauze to obtain spore suspension bacterial agent, use sterile water Adjust the number of spores in the spore suspension to 10 4 individual / mL.

[0039] The pear branch powder medium is: 10 g of pear branch powder, 10 g of inorganic nutrient solution, the inorganic nutrient solution is evenly added to the pear branch powder to a wet state, and autoclaved at 121° C. for 20 minutes.

[0040] The pear branch powder is dried to constant weight, crushed and passed through a 20-mesh sieve.

[0041] The preparation method of described inorganic nutrient solution is: NH 4 Cl2.0g, MgSO 4 ·7H 2 O0.5g, KH 2 PO 4 1.0g, Na 2 HPO 4 0.2g, MnSO 4 0.0035g, CuSO 4 ·5H 2 O0.007g, FeSO 4 ·7H 2 O0.007g, ZnSO 4 0.002g, CoCl 2 ...

Embodiment 3

[0042] Example 3 Determination of lignin-degrading enzyme activity of bacterial strain D10

[0043] Bacterial strain D10 is inoculated into the Erlenmeyer flask that 50mL pear tree branch powder culture medium (same as Example 2) is housed, under the condition of 30 ℃ static culture 7 days, add 30mL sterile water to Erlenmeyer flask, be placed on the shaker shaker 30min , obtain the spore suspension bacterial agent after filtering the culture with gauze, adjust the number of spores in the spore suspension bacterial agent to be 10 with sterile water 4 individual / mL. Inoculate 1% of the above-mentioned spore suspension agent into 100mL in a conical flask (250mL) of a liquid enzyme-producing medium with pear tree branch powder as the only carbon source, place at 30°C for 160r / min shaking culture for 21 days, and take samples every 2 days Once, the fermentation broth was centrifugally filtered to remove bacteria and solid impurities to obtain a crude enzyme solution, and the acti...

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Abstract

The invention belongs to an agricultural waste recycling technology and discloses a degrading fungus capable of decomposing trimmed pear branches and a fungicide of the degrading fungus. The strain D10 belongs to Ceriporia lacerata which is preserved with the number of CGMCC NO.7852 in China general microbiological culture collection center on July 8th, 2013. The strain can grow on a culture medium with a pear branch as the unique carbon source. The maximum enzyme activity values of laccase, lignin peroxidase and manganese peroxidase of the strain D10 are respectively 11.03U.Ml<-1>, 36.71U.Ml<-1>and 152.75U.Ml<-1> in the 21d liquid fermentation process. According to the invention, agricultural waste trimmed pear branches are degraded by using a microbial fermentation method and are converted into an organic fertilizer, so that wastes are turned into wealth, the environment is protected, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the intensive agricultural production technology, and relates to a pear tree branch degrading fungus and its bacterial agent, which are specially used for degrading discarded pear tree branches to produce organic fertilizers and realize the resource utilization of organic waste. Background technique [0002] Pruning is an important technical measure in the cultivation and management of pear trees. Proper pruning has a positive impact on the growth of fruit trees and the yield and quality of fruits. For pear orchards in full fruit stage, the amount of pruned branches is generally 1500-2250kg·hm -2 . Statistics from the Department of Market and Economic Information of the Ministry of Agriculture show that in 2011, the cultivated area of ​​pear trees in my country was more than 1.1 million hectares. According to this figure, the annual pruning of pear orchards in my country can reach 1.61-2.42 million tons. The branches are rich...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F17/00C05F11/08C12R1/645
CPCY02W30/40
Inventor 徐阳春张乃文韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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