Aspergillus niger NY-1 as well as improvement technique for improving insecticidal activity of aspergillus niger NY-1 and application of aspergillus niger NY-1

Abstract

The invention relates to epigenetic inheritance modification, and in particular relates to an aspergillus niger NY-1 as well as an improvement technique for improving the insecticidal activity of the aspergillus niger NY-1 and application of the aspergillus niger NY-1. The aspergillus niger NY-1 was collected in China Center for Type Culture Collection (CCTCC) on April 28th, 2014 with the collection number CCTCC NO: M2014169. The chemical epigenetic inheritance modification technique is used for improving the insecticidal activity of an NY-1 fermentation product and is applied to a microbial pesticide. The strain is separated from burdock collected in Anqiu, Shandong; an ITS zone is amplified through PCR, and the sequencing result shows that the similarity of the strain is 100% compared with a strain Aspergillus niger (Accession number: KF358715.1) in a Genebank database. An insecticidal silent gene of the aspergillus niger NY-1 is activated by adopting a chemical epigenetic modifier, so that the insecticidal activity of the NY-1 is improved, and the NY-1 is applied to the microbial pesticide. Compared with gene control and other silent gene activation technologies, the method has the advantages of quickness, low cost, obvious effect and the like.

Description

technical field [0001] The invention relates to chemical epigenetic modification technology, more specifically, an Aspergillus niger NY-1 strain and the improvement technology and application of improving its insecticidal activity. After strain NY-1 is treated with chemical epigenetic modifiers, the extract of its fermentation broth has higher insecticidal activity. Background technique [0002] Symbiotic fungi exhibit abundant secondary metabolism capabilities, and some secondary metabolites also exhibit insecticidal activity. However, when the endophytic bacteria fermentation method is used to produce natural insecticides, on the one hand, after the endophytic bacteria leave the plant, Secondary metabolism is often stopped or delayed; on the other hand, endophytes have different metabolic pathways under autobiotic and symbiotic conditions. When Bok J.W et al. studied the genome of Aspergillus nidulans, they found that there were 1 terpene, 2 indole alkaloids, 14 non-ribos...

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Problems solved by technology

[0002] Symbiotic fungi exhibit abundant secondary metabolism capabilities, and some secondary metabolites also exhibit insecticidal activity. However, when the endophytic bacteria fermentation method is used to produce natural insecticides, on the one hand, after the endophytic bacteria leave the plant, Secondary metabolism is often stopped or delayed; on the other hand, the metabolic pathways of endophytes will be different under autobiotic and symbiotic conditions

When Bok J.W et al. studied the genome of Aspergillus nidulans, they found that there were 1 terpene, 2 indole alkaloids, 14 non-ribosomal polypeptides and 27 polyketides in the genome of the fungus. The gene exists, but when it is fermented and cultivated under experimental conditions, only a few secondary metabolites can be expressed, which seriously hinders the research and development of fungal natural medicines (Chem Biol, 2006, 13(1): 31-37. )

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