Probe capable of specifically identifying bisphenol A nucleic acid aptamer and test strip detection application of probe

Abstract

The invention discloses a probe capable of specifically identifying bisphenol A nucleic acid aptamer and test strip detection application of the probe. The probe has a sequence shown in SEQID NO.1, and can be applied to the preparation of a bisphenol A immunochromatographic gold-labeled test strip, and the like; the test strip comprises a lining plate; at least four sticking objects including a sample film, a nucleic acid aptamer probe-gold-labeled conjugate pad, an enveloping film provided with a detection line and a reference line, and a water absorption film are sequentially stuck on the lining plate, wherein the nucleic acid aptamer probe-gold-labeled conjugate pad comprises compound of a nucleic acid aptamer probe and gold nanoparticles. Due to the test strip, the rapid qualitative and quantitative determination of bisphenol A can be realized by direct observation without using other auxiliary instruments and equipment, the detection work can be rapidly completed, and the accurate detection result can be obtained; the probe capable of specifically identifying the bisphenol A nucleic acid aptamer has the characteristics of being high in specificity, good in stability, low in cost, easy to use and the like, and can be used for a hospital, a family, the customhouse with responsibility of food safety inspection and quarantine, an airport, a port and the like which need a rapid field detection mechanism.

Description

technical field [0001] In particular, the present invention relates to a specific recognition bisphenol A nucleic acid aptamer probe and its application in detecting bisphenol A, for example, a bisphenol A immunochromatographic gold standard test strip based on the nucleic acid aptamer probe , its preparation method and application. technical background [0002] With the development of science and technology and the increasing requirements for the convenience and speed of life, plastic products have been widely used in all aspects of life. Bisphenol A is an important component of synthetic polycarbonate and epoxy resins, which are used to make plastic (milk) bottles, suction cups for children, and coatings on the inside of food and beverage (milk powder) cans. According to research, bisphenol A can lead to endocrine disorders, induce precocious puberty, and threaten the health of fetuses and children. At the same time, cancer and obesity caused by metabolic disorders are al...

AI Technical Summary

Problems solved by technology

Although relying on traditional instruments for detection has the advantages of high detection sensitivity and good test result stability, large-scale instruments are usually expensive and require professional operators with relevant knowledge. At the same time, there are disadvantages such as high detection costs, which limit the large-scale instruments Scale detection and on-site rapid detection

The enzyme-linked immunosorbent assay based on immunoassay can solve the shortcomings of high detection cost of large-scale instruments and cannot be used for on-site detection, but because the enzyme-linked immunosorbent assay requires multiple steps of repeated washing and separation processes in the detection process, It requires people with certain professional knowledge to operate, which also limits the widespread use of this method to a certain extent

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