Method for breeding onion male sterile line and maintainer line by utilizing molecular markers

Abstract

The invention discloses a method for breeding onion male sterile line and maintainer line by utilizing molecular markers. A PCR (polymerase chain reaction) experiment is performed to detect single onion ball nucleoplasm genotype by utilizing specific primers (disclosed as SEQ ID NO.1-6) to screen a sterile strain and a maintainer strain onion ball, thereby implementing two-line matching. A plurality of sets of primers are designed according to Ms allele flanking sequences (F1890 and S1887) and onion cytoplasmic male sterility specific sequence mt472; and scientific experiments are performed to develop a molecular marker detection system and detection kit based on multiplex PCR. The detection system can distinguish 6 karyoplasm fertility types of onions through one PCR experiment; and the detection system is utilized to directly breed the sterile line and maintainer line from the open pollination colony, thereby implementing one-step matching of two lines. The development of the multiplex PCR detection system has important meanings in accurate and quick identification of onion nucleo-cytoplasmic interreaction male sterility karyoplasm genotype.

Description

technical field [0001] The invention relates to a method for breeding onion male sterile lines and maintainer lines by using molecular markers, and belongs to the field of biotechnology. Background technique [0002] Onion (Allium cepa L.) belongs to Allium genus Allium (APG III classification system), a biennial vegetable crop with a cultivation history of more than 5,000 years. It is a worldwide vegetable, and onions are cultivated in at least 139 countries. According to FAO statistics in 2012, the world's onion planting area is 4.2 million hectares, with an output of 82.9 million tons. Among vegetable crops, its output ranks third in the world. my country has the world's largest onion production area and output, accounting for about 30% of the world's total planting area and output. Onions are resistant to storage and transportation, not only can adjust the supply of vegetables, but also an important vegetable for export and foreign exchange. From the perspective of nut...

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Problems solved by technology

There is no report on the nuclear-plasma co-detection system, and there is no report on the application of this system to the screening and matching of sterile lines and maintainer lines.

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