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Special chip for detecting breast cancer related gene expression

A special chip and gene expression technology, applied in the field of special chips for detecting breast cancer-related gene expression, can solve the problems of cumbersome operation, inconvenient automatic integration, etc., and achieve the effect of shortening detection time and convenient expression screening.

Active Publication Date: 2021-12-28
BEIJING BIONAXIN BIOTECH CO LTD
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Problems solved by technology

[0006] The current main method is to extract RNA from patient tissue slices. A series of complex operations such as dehydration and hydration are required to obtain purified RNA, and the obtained RNA can be added to each PCR tube according to the test target. The PCR tube completes the quantitative PCR reaction of a gene (RNA). The operation is very cumbersome and requires professionals to complete, which is not convenient for automatic integration.

Method used

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  • Special chip for detecting breast cancer related gene expression
  • Special chip for detecting breast cancer related gene expression

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Embodiment 1

[0025] A special chip for detecting breast cancer-related gene expression, characterized in that it comprises the following steps:

[0026] S1 According to the mature mRNA sequence of the breast cancer 21 gene specified in the breast cancer guidelines, design the corresponding amplification primers, see the table below for details:

[0027] Table 1 Primers for breast cancer 21 gene expression detection

[0028]

[0029] Screen genes related to breast cancer occurrence and breast cancer prognosis according to the relevant guidelines for breast cancer treatment, and select a pair of probes for each gene according to the specific mRNA sequence of each gene to ensure that the set of probe pairs can complete the specific mRNA RT-PCR amplification of the sequence;

[0030] S2 completes the chemical synthesis of the probe pair, and at the same time ensures that the 5' end of each probe is added with a connecting arm composed of 6 ethylene glycol polymer chains, and the end is con...

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Abstract

The invention relates to a special chip for detecting breast cancer related gene expression. A pair of primers for RT-PCR is used as probes to be fixed on a substrate, a gene chip is used for completing simultaneous detection of multi-gene expression profiles at one time, and the detection of breast cancer specific gene expression conditions can be rapidly completed by using an RNA sample.

Description

technical field [0001] The invention relates to the field of biological chips, in particular to a special chip for detecting the expression of breast cancer-related genes. Background technique [0002] Biochip technology has been widely used in clinical disease diagnosis, health management, drug research and development, animal and plant quarantine, food testing, environmental monitoring, scientific research, forensic testing and many other fields. It has broad application prospects and a very large market demand. [0003] Gene chip is a type of biochip, which is prepared by planting a series of probes with known sequences on the chip substrate, which can be used for hybridization detection of specific labeled nucleic acids, and can report the number of detected objects through identification, detection and information processing. Nucleic acid information. [0004] In 2020, there will be 19.29 million new cancer cases worldwide, including 10.06 million males and 9.23 millio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6837
CPCC12Q1/6886C12Q1/6837C12Q2600/158C12Q2600/118C12Q2565/519C12Q2565/537C12Q2531/113C12Q2563/107
Inventor 华子昂刘宝全竹添朱美瑛万君兴张建李娜
Owner BEIJING BIONAXIN BIOTECH CO LTD
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