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Identification method for fingerprint spectrum of astragalus medicinal material

A technology of fingerprints and astragalus, applied in the field of identification of fingerprints of astragalus medicinal materials, can solve the problems of few chemical components, small amount of information, and small number of components, so as to enhance immune function, reduce the interference of the spectrum, and enhance myocardial contractility Effect

Active Publication Date: 2014-08-06
XIAN SHIJISHENGKANG PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The 2010 edition of "Chinese Pharmacopoeia" uses thin-layer chromatography to identify Astragalus membranaceus. Since the TLC identification method itself is affected by many factors such as temperature, humidity, thickness of the thin-layer plate, and sample load, the chemical composition of the sample is not fully reflected. The number of identifiable components is small, and the reproducibility of the same sample is not good. However, the Astragalus fingerprint technology reported in the literature has fewer chemical components and provides a small amount of information, which cannot represent Astragalus well. The overall quality of medicinal materials cannot effectively identify the quality of Astragalus medicinal materials

Method used

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  • Identification method for fingerprint spectrum of astragalus medicinal material
  • Identification method for fingerprint spectrum of astragalus medicinal material
  • Identification method for fingerprint spectrum of astragalus medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Determination of fingerprints of Radix Astragali medicinal materials

[0029] 1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0030]Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Verbacosin, formononetin, and formononetin reference substances were purchased from China Biological Products Co., Ltd. All samples are reserved in the Sample Room of the Modernization Center of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

[0031] 2. Chromat...

Embodiment 2

[0056] Embodiment two: Astragalus medicinal material fingerprint chromatogram determination

[0057] 1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0058] Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Verbacosin, formononetin, and formononetin reference substances were purchased from China Biological Products Co., Ltd. All samples are reserved in the Sample Room of the Modernization Center of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

[0059] 2. C...

Embodiment 3

[0067] Embodiment 3: Astragalus medicinal material fingerprint chromatogram determination

[0068] 1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0069] Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Verbacosin, formononetin, and formononetin reference substances were purchased from China Biological Products Co., Ltd. All samples are reserved in the Sample Room of the Modernization Center of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

[0070] 2. Chr...

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Abstract

The invention provides an identification method for a fingerprint spectrum of an astragalus medicinal material. The method consists of: preparation of a test solution, measurement of a fingerprint spectrum, determination of a control fingerprint spectrum, and identification of an astragalus medicinal material fingerprint spectrum. By high performance liquid chromatography (HPLC) fingerprint spectrum measurement and analysis on the astragalus medicinal material, 9 characteristic peaks of the astragalus medicinal material can be determined, and the common characteristic peaks constitute fingerprint features of the astragalus medicinal material and can serve as the control fingerprint spectrum of the astragalus medicinal material. The astragalus medicinal material needing identification can be compared with the control fingerprint spectrum to inspect the situation of the common characteristic peaks so as to identify quality. With the characteristics of simplicity, good repeatability, obvious characteristic peaks, accuracy and reliability, etc., the method provided by the invention is conducive to identifying the quality of the astragalus medicinal material.

Description

technical field [0001] The invention relates to a method for identifying the fingerprints of astragalus medicinal materials, in particular to a method for identifying the quality of effective components of astragalus medicinal materials by using high performance liquid chromatography fingerprint method. Background technique [0002] Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao or Astragalus membranaceus (Fisch) Bge. Astragalus contains flavonoids and saponins; Methoxy isoflavone, 7,2'-dihydroxy-3',4'-dimethoxy isoflavane and its glucoside, 7,3'-dihydroxy-4',5'-dimethoxy Isoflavane, 3-hydroxy-9,10-dimethoxy pterostane and its glucoside, etc.; saponins include astragaloside Ⅰ~Ⅷ and soybean saponin Ⅰ; astragaloside IV (astragaloside IV) and astragaloside B glycosides. Astragaloside IV (C 22 h 22 0 10 ), calycosin glucoside (C 22 h 22 o 10 ) as an index for content determination; Li Yan established a high performance liquid chromatography method to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 果德安吴婉莹赵丙贤吕延英刘蓉霞姚帅王俊平赵兴蔡录影王丹丹侯晋军笪娟
Owner XIAN SHIJISHENGKANG PHARMA IND
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