In-vitro stress culture device for blood vessel, culture system and culture method

Abstract

The invention discloses an in-vitro stress culture device for a blood vessel, a culture system and a culture method. The device is characterized in that two sides of a culture tank are coaxially provided with connecting pieces in which first fixed parts are coaxially arranged, fasteners are coaxially fixed outside the connecting pieces and are internally and coaxially provided with second fixed parts, and the first fixed parts and the second fixed parts are internally, respectively and coaxially provided with a third through hole and a fourth through hole; sealing rings are arranged between the first fixed parts and the second fixed parts; blood vessel connecting pipes extend into the culture tank after passing through the fourth through holes, the sealing rings and the third through holes. The device is capable of improving the coaxiality of the blood vessel and the blood vessel connecting pipes and good in sealing performance. A surge flask in the system is formed into a loop together with the in-vitro stress culture device for the blood vessel through an input pipeline and an output pipeline; the input pipeline is provided with a constant flow pump and a flow sensor, and the output pipeline is provided with a pressure sensor; a gas regulating valve, the constant flow pump, the flow sensor and the pressure sensor are respectively connected with a PC (personal computer). The system and the culture method can be used for precisely controlling the pressure and flow in a culture process and improving the reliability of a culture result.

Description

technical field [0001] The invention belongs to the field of in vitro culture of blood vessels, and in particular relates to an in vitro stress culture device, a culture system and a culture method for blood vessels. Background technique [0002] In a normal physiological environment, arteries are always under the action of blood pressure and blood flow. The mechanical stress caused by blood pressure and blood flow, that is, circumferential stress and shear stress, always acts on the vessel wall. When these mechanical stresses are altered, they lead to adaptive changes in the function and structure of blood vessels. Vascular remodeling under hypertensive conditions may be an adaptive response to increased mechanical stress. [0003] The isolated arterial vessel culture device began to appear in the 1990s. In 1995, Nathalie Bardy et al. designed a vascular organ culture model in vitro. The thoracic aorta of rabbits was cultured in vitro. The artery survived for more than 8 ...

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Problems solved by technology

In 1996, Robert et al. used dog common carotid artery and human saphenous vein to culture blood vessels in vitro. Their research focused on the pulsating fluid pressure, and the design of the blood vessel culture chamber was flawed, which could not withstand long-term culture and could not control the steady flow. (Robert F Labadie, et al. Pulsatile perfusion system for ex vivo investigation of biochemical pathways in intact vascular tissue. Am J Physiol, 1996, 270 (Heart Circ Physiol39): H760.)

In 2001, Han Haichao et al. designed a similar vascular culture system in vitro to study the systolic responsiveness of cultured porcine common carotid arteries in vitro under the action of pulsating high pressure. and flow computer control (Han HC, Ku DN. Contractile responses in arteries subjected to hypertensive pressure in seven-day. Organ culture, Annals of Biomedical Engineering, 2001, 29: 467.)

In the culture of small animal blood vessels, there is also the problem that the culture tank and the connecting conduit are not well fixed to each other. For example, the connecting conduits fixed at both ends of the culture tank have poor coaxiality (that is, they are not on the same line in space), and there is inconvenient installation. and the disadvantages of leakage

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