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Preparation and application of anaerobic ammonia oxidizing bacteria immobilized star-shaped bioactive filler based on reticulate carrier

An anammox and biological activity technology, which is applied in the field of preparation of anammox bacteria immobilized star-shaped biologically active fillers, can solve the problems of easy detachment of the embedded body, poor overall stability, and inability to be ideally applied, and achieves improved affinity. Water-based, not easy to fall off, the effect of increasing the strength of the bond

Active Publication Date: 2014-07-30
湖南汨罗循环经济产业园区科技创新服务中心
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

This method is simple to operate, but has great limitations and cannot be ideally applied to engineering practice
Experimental studies have shown that the bioactive fillers prepared by combining traditional carriers such as Raschig rings, Pall rings, stepped rings, and suspended modified fillers with embedding bodies through adhesion have poor overall stability and embedding The body is easy to fall off, causing problems such as bacterial loss

Method used

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  • Preparation and application of anaerobic ammonia oxidizing bacteria immobilized star-shaped bioactive filler based on reticulate carrier
  • Preparation and application of anaerobic ammonia oxidizing bacteria immobilized star-shaped bioactive filler based on reticulate carrier
  • Preparation and application of anaerobic ammonia oxidizing bacteria immobilized star-shaped bioactive filler based on reticulate carrier

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Embodiment Construction

[0028] The present invention will be further specifically described below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0029] 1. Preparation of star-shaped mesh carriers

[0030] Using polypropylene as the main material, add polyvinyl alcohol (PVA) with a mass fraction of 5% to make a sheet-shaped mesh carrier with a length of 1.0 cm, a width of 0.5 cm, and a wire diameter of 1 mm. Three sheets of the above-mentioned sheet-shaped carriers are bonded by heat fusion to form a star-shaped mesh carrier with an included angle of 120°.

[0031] 2. Treatment of Anammox Bacteria

[0032] The cultured anammox bacterial suspension was centrifuged and concentrated to obtain a bacterial concentration of 3.0×10 10 A / mL concentration of anammox bacteria.

[0033] 3. Acquisition of Anammox Bacteria Embedding Solution

[0034] Weigh 10.0g of polyvinyl alcohol, add 70ml of ultra-pure water, put it into a high-temperature sterilizatio...

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Abstract

The invention discloses an anaerobic ammonia oxidizing bacteria immobilized star-shaped bioactive filler based on a reticulate carrier, and preparation and an application thereof, and belongs to the technical field of water treatment. The bioactive filler comprises two parts: an embedding body and the carrier; the carrier is a reticulate star-shaped carrier formed by taking polyethylene, polyvinyl chloride, polypropylene or ABS resin as a main material, adding polyvinyl alcohol and carrying out hot melting or plate hot pressing; an embedding liquid is formed by mixing an anaerobic ammonia oxidizing bacteria suspension and a polyvinyl alcohol solution and evenly coats the reticulate carrier; the embedding body is formed after boric acid secondary cross-linking immobilization of the embedding liquid and is combined onto the reticulate carrier to obtain the anaerobic ammonia oxidizing bacteria bioactive filler. With a reticulate carrier structure of the prepared bioactive filler, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure so as to increase the filler overall stability; the filler can still maintain a loose state, problems of blockage, water flow short-out and the like cannot appear, the concentration of anaerobic ammonia oxidizing bacteria on the filler is high, the treatment efficiency of the bioactive filler is high, and the performance is stable.

Description

technical field [0001] The invention relates to the field of water treatment, in particular to the preparation and application of anaerobic ammox bacteria-immobilized star-shaped bioactive filler based on a mesh carrier. Background technique: [0002] Anaerobic ammonium oxidation technology (ANAMMOX) is currently known as the most economical biological denitrification pathway. Studies by Strous et al. at Delft University in the Netherlands have shown that the biological reaction process including cell synthesis in the anaerobic ammonium oxidation process can be expressed as the formula ( 1) [0003] NH 4 + +1.32NO 2 -+0.066HCO 3 ‐ +0.13H + → [0004] 1.02N 2 +0.26NO 3 -+0.066CH 2 o 0.5 N 0.5 +2.03H 2 O(1) [0005] From the biological reaction characteristics of anammox bacteria, it can be seen that compared with traditional nitrification and denitrification technology, ANAMMOX has the advantages of low operating cost, low oxygen demand and no need for additiona...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F3/34
Inventor 杨宏陶慕翔王猛管清坤陈伟鄢琳王小乐吴城锋孟婷尚海源胡希佳赵月兰王玉洁姚仁达
Owner 湖南汨罗循环经济产业园区科技创新服务中心
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