Method for preparing nano-liposome marked by colloidal gold and loaded with PD (pharmacodynamics) medicine
A nano-liposome and colloidal gold technology, applied in the field of biological sciences, can solve problems such as difficult to cross the BBB, poor brain targeting, macrophage phagocytosis, etc., to achieve small particle size, high encapsulation efficiency, and good stability Effect
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Embodiment 1
[0023] Example 1 A method for preparing colloidal gold-labeled L-dopa nanoliposomes (the encapsulation rate of L-dopa is 62.52%, and the encapsulation rate of colloidal gold is 72%)
[0024] according to m 大豆卵磷脂 :m 胆固醇 = 8:1, weigh soybean lecithin and cholesterol, dissolve in absolute ethanol; according to m L-dopa :m 胶体金 :m 大豆卵磷脂 = 20:1:2000, absorb the corresponding L-dopa mother solution and colloidal gold mother solution, add a certain amount of PBS (pH = 7.4) solution to dissolve. Add the PBS solution of colloidal gold L-dopa into a 20mL beaker, place it in a water bath at 45~55°C, add lecithin and cholesterol absolute ethanol solution dropwise while stirring, and continue stirring at constant temperature until the ethanol evaporate completely. The liposome suspension was ultrasonically treated in a water bath for 10 min to obtain a pale pink liposome suspension, and the liposome suspension was slowly injected into a centrifuge tube equipped with a sucrose density ...
Embodiment 2
[0034] Example 2 A method for preparing colloidal gold-labeled amantadine hydrochloride nanoliposomes (the encapsulation rate of amantadine hydrochloride is 56.54%, and the encapsulation rate of colloidal gold is 76.78%)
[0035] according to m 大豆卵磷脂 :m 胆固醇 = 8:1, weigh soybean lecithin and cholesterol, dissolve in absolute ethanol; according to m 盐酸金刚烷胺 :m 胶体金 :m 大豆卵磷脂 = 20:1:1200, absorb the corresponding amantadine hydrochloride mother solution and colloidal gold mother solution, add a certain amount of PBS (pH = 7.4) solution to dissolve. Add the PBS solution of colloidal gold amantadine hydrochloride into a 20mL beaker, place it in a water bath at 45~55°C, add the absolute ethanol solution of lecithin and cholesterol dropwise while stirring, and continue stirring at constant temperature until Ethanol evaporates completely. The liposome suspension was ultrasonically treated in a water bath for 10 min to obtain a pale pink liposome suspension, and the liposome suspens...
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