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Method for extracting refined ursolic acid from loquat leaves

An extraction method and loquat leaf technology are applied in the field of natural plant extract preparation, can solve problems such as unfavorable industrial application, increase production equipment, increase production cost, etc., and achieve the effects of being beneficial to industrial application, reducing production process and facilitating filtration

Active Publication Date: 2014-07-16
CHANGSHA LUYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, preliminary research on the separation and purification of ursolic acid in loquat leaves has been carried out at home and abroad, but the existing method steps are relatively cumbersome, the extraction rate is low, and the cost is too high, which not only prolongs the production time, increases the production equipment, but also greatly increases Production cost, not conducive to industrial application

Method used

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  • Method for extracting refined ursolic acid from loquat leaves

Examples

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Effect test

Embodiment 1

[0013] Embodiment 1: the method for extracting ursolic acid from loquat leaf described in the present invention, comprises the following steps:

[0014] (1) Take 2Kg of fresh or sun-dried loquat leaves, crush them, and pass through a 10-12 mesh sieve; then mix the crushed loquat leaves with ethanol and Span-85 for ultrasonic extraction, and Span-85 The addition amount is 0.1% of the total weight of the pulverized loquat leaves and ethanol. When extracting, use 8-10 times of 95% ethanol solution to extract twice, and the extraction temperature is 70-80°C; after filtering off the medicinal residue, combine the filtrate, pass through a microporous filter membrane with a pore size of 0.7 μm, and use a filter with a molecular weight cut-off of 4000Da for the filtrate. Ultrafiltration membrane is used for ultrafiltration to obtain ultrafiltrate;

[0015] (2) Concentrate the ultrafiltrate until the relative density of the solution is 1.1-1.2g / ml, carry out vacuum drying, control the...

Embodiment 2～7

[0017] Examples 2-7: The difference from Example 1 is that the amount of Span-85 or Span-65 is different, the settings of the microporous membrane pore size and ultrafiltration membrane particle size are different, and the product purity and extraction rate are different. See the table below for details. The rest are the same and will not be repeated here.

[0018] Content detection result table of each embodiment

[0019]

[0020] The production method of the control group:

[0021] (1) Take 2Kg of fresh or sun-dried loquat leaves, crush them, and pass through a 10-12 mesh sieve; then carry out reflux extraction of the crushed loquat leaves, and extract twice with 8-10 times 95% ethanol solution during extraction , the extraction temperature is 70-80°C; after the medicinal residues are filtered out, the filtrate is combined, and the filtrate is vacuum-concentrated to a solid-liquid ratio of 1︰0.7-1.2, the vacuum degree is controlled to be ≥0.07Mpa, the temperature is 50°...

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Abstract

The invention discloses a method for extracting a refined ursolic acid from loquat leaves. The method comprises the following steps: evenly mixing crushed and sieved loquat leaves with any one of ethanol, Span-65 and Span-85 and carrying out ultrasonic extraction, performing microfiltration and ultrafiltration on the extract, and then concentrating and drying to obtain crude ursolic acid; crushing, eluting, decoloring, filtering, concentrating and precipitating the crude ursolic acid, and carrying out suction filtration to obtain white precipitate; next, adding ethanol, heating until boiling, and chilling until complete crystallization; further concentrating the mother liquor and operating by use of the same method to obtain secondary crystals, blending the crystals of the two times, and drying in vacuum to obtain the refined ursolic acid. The method has the advantages that maximum separation and purification effect can be achieved, impurities are removed effectively, the purity of the ursolic acid is increased and the extraction rate is above 25%; the method is simplified in production step, reduced in production cost, reasonable in technology, safe to operate, environmental friendly, and advantageous for industrial application.

Description

technical field [0001] The invention relates to the field of preparation of natural plant extracts, in particular to a method for extracting refined ursolic acid from loquat leaves. Background technique [0002] Ursolic acid (UA) is also known as ursolic acid and ursolic acid, and its molecular formula is C 30 h 48 o 3 , is an ursane-type triterpene, which belongs to the α-amyresinol-type pentacyclic triterpenoid compound, and is widely distributed in the plant kingdom, mainly loquat leaves and hawthorn leaves. Ursolic acid is white needle-like crystal, melting point 227-228℃, easily soluble in dioxane and pyridine, soluble in methanol, ethanol, butanone, slightly soluble in acetone, slightly soluble in benzene, chloroform, ether, insoluble in water and petroleum ether. [0003] Ursolic acid has a wide range of biological activities. Modern pharmaceutical research shows that ursolic acid has clinical pharmacological effects such as anti-inflammatory, enhancing immunity, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 谢君
Owner CHANGSHA LUYUAN BIO TECH
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