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Composition for treating psoriasis and preparation method thereof

A psoriasis and compound technology, applied in the field of preparation of nano liposome gel (AKBA-Lip for short), can solve the problem of high production cost of psoriasis drugs, affecting AKBA bioavailability, AKBA cannot be taken orally and Injection and other problems, to achieve the effect of strong targeting, significant effect, and good skin penetration

Active Publication Date: 2014-07-02
SUZHOU BOTANY BIOMEDICALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In general, the existing drugs for the treatment of psoriasis have high production costs and poor targeting. Although 11 carbonyl-β-acetylboswellic acid has potential excellent uses, AKBA is a fat-soluble substance and is difficult to dissolve in water. In the study, the drug was mainly dissolved in DMSO. When the experimental ratio required for the dilution of the mother solution was prepared with DMSO, flocs were likely to precipitate, which would affect the bioavailability of AKBA. In addition, AKBA dissolved in DMSO could not be taken orally. Injection, which greatly limits its clinical use

Method used

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  • Composition for treating psoriasis and preparation method thereof
  • Composition for treating psoriasis and preparation method thereof
  • Composition for treating psoriasis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0062] Example 1: AKBA Extract Preparation

[0063] Separation of AKBA by silica gel column chromatography:

[0064] Take frankincense extract as raw material, dissolve it in methanol, mix the raw material with column chromatography silica gel (200-300 mesh) at a ratio of 1:5, and mix the sample to dry. The dry method is used to load the sample, and the sample volume is 1:15 (w / w). Elute with methanol-dichloromethane (1:100), and collect the eluate, each 50ml is a bottle, use the AKBA standard as a control, detect the collected solution with TCL, and combine the collected solution that is the same as the standard Rf. Concentrate under reduced pressure and then pass through the silica gel column at the loading ratio of 1:30 and 1:60, and the white powder dried under reduced pressure is the crude extract of AKBA.

[0065]

[0066] The crude extract was purified by preparative HPLC:

[0067] Chromatographic conditions: the mobile phase is chromatographic methanol-ultrap...

Embodiment 2

[0073] Example 2: AKBA-Lip preparation ( figure 2 )

[0074] Compound of molecular formula (I) 0.1 g (or 1.5 g, 2.0 g)

[0075] Soy lecithin 0.8 g

[0076] Cholesterol 0.3 g

[0077] VE 0.1g

[0078] Poloxamer 188 0.2 g

[0079] Kabomu U20 1.5 g

[0080] Propylene glycol (2%) 2 g

[0081] Glycerin (2%) 2 g

[0082] Preservatives (0.5%) 0.5 g

[0083] Appropriate amount of triethanolamine (adjust PH value)

[0084] water to 100 g

[0085] The preparation method of described composition is characterized in that comprising the following steps:

[0086] A Preparation of liposomes: Weigh soybean phospholipids, cholesterol, AKBA, VE according to the prescription and dissolve them in chloroform and absolute ethanol mixed solvent, transfer them to a 250ml round bottom flask, add PBS (pH 5.8, containing poloxa Mu 188 water-soluble components) form a two-phase system. A uniform W / O emulsion was formed by ultrasonication on a water bath <10°C, and the W / O emulsion was re...

Embodiment 3

[0090] Example 3: The test of AKBA-Lip inhibiting NF-kappaB in vitro ( image 3 )

[0091] Take a certain amount of AKBA liposome and dilute it in 1XPBS to prepare a 500umol solution. The macrophage cell line RAW 264.7 was stimulated with 100ng / mL LPS for six hours with and without AKBA liposomes (concentration: 75umol) and extracted for Western Blooting analysis. By analyzing phosphorylated IkBα (abbreviation: Phospho-IkB), it was found that 75umol of AKBA effectively inhibited the activation of NF-kappaB.

[0092]

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Abstract

The invention discloses a natural compound for treating psoriasis, a composition of the compound, and a preparation method and application of a nano liposome gel (referred to as: AKBA-Lip) of the compound and the composition.

Description

technical field [0001] The invention relates to a natural compound for treating psoriasis, a composition of the compound, and a preparation method and application of the nano liposome gel (abbreviated: AKBA-Lip) of the compound and the composition. [0002] Background technique [0003] Psoriasis is a common chronic, relapsing, inflammatory skin disease characterized by recurrent multilayered silvery white dry scales on erythema. In ancient Chinese medicine, it was called "white phlegm", and in ancient medical books, it was also called tinea pine. Western medicine is called psoriasis, commonly known as psoriasis, which is characterized by pimples and erythema of various sizes, covered with silvery white scales on the surface, with clear boundaries, and occurs on the scalp, extremities and back. It is easy to recur or aggravate in spring and winter, and it is more relieved in summer and autumn. According to investigations, the incidence of psoriasis accounts for 0.1%-3% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K31/56A61K9/127A61P17/06
Inventor 不公告发明人
Owner SUZHOU BOTANY BIOMEDICALS
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