Medicinal application of chicoric acid
A technology of cichoric acid and medicine, which is applied in the field of cichoric acid as histone deacetylase inhibition, and can solve the problem of not being able to promote virus resistance
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Embodiment 1
[0025] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed on H4IIE cells using cell lysates:
[0026] Inoculate H4IIE cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of the well is covered with cells. Add different concentrations (5, 10, 20, 100 μM) of cichoric acid. 4h and 24h, prepare the whole cell lysate, use the bicinchoninic acid method to determine its protein content, take the cell lysate without adding cichoric acid as the blank, and use the cell lysate with sodium butyrate as the positive control, according to Merck Millipore The method described in the instruction manual of the Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) produced by the company was used to determine the activity of histone deacetylase, and the results are as follows:
[0027] The inhibitory effect of cichoric acid on HDAC was determined by H4IIE cell lysat...
Embodiment 2
[0032] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed in H4IIE cells using nuclein:
[0033] Inoculate H4IIE cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of the well is covered with cells. , 24h, extract the nuclear protein of the cells, use the bicinchoninic acid method to measure its protein content, take the nuclear protein without adding cichoric acid as a blank, and take the nuclear protein with sodium butyrate as a positive control, according to the production of Merck Millipore The method described in the instructions of the Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) was used to determine the activity of histone deacetylase, and the results were as follows:
[0034] The inhibitory effect of cichoric acid on HDAC was determined by H4IIE nucleoprotein (cells were treated for 2h)
[0035] medicine
Inhibition...
Embodiment 3
[0041] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed on L6 cells using cell lysates:
[0042] Inoculate L6 cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of the well is covered with cells. , 24h, prepare the whole cell lysate, use the bicinchoninic acid method to measure its protein content, take the cell lysate without adding cichoric acid as the blank, and take the cell lysate with sodium butyrate as the positive control, according to Merck Millipore The method described in the instruction manual of the production of Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) was used to measure the activity of histone deacetylase, and the results are as follows:
[0043] The inhibitory effect of cichoric acid on HDAC was determined by L6 cell lysate (cells were treated for 4h)
[0044] medicine
Inhibition rate(%)
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