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Biocontrol bacteria, separation method, bacterial agent and application thereof for preventing and treating sesame fusarium wilt

A technology for sesame fusarium wilt and biocontrol bacterial agent, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., and can solve the problem that the proportion of high resistance to fusarium wilt materials is low, the control effect is not obvious enough, and the sesame fusarium wilt has no effect. Better prevention and control of technical methods and other issues to achieve the effect of promoting growth and obvious growth-promoting effect

Active Publication Date: 2015-09-30
HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the narrow genetic basis of sesame germplasm resources, no sesame germplasm resources that are immune to Fusarium wilt have been found so far, and the proportion of materials with high resistance to Fusarium wilt is also low. In addition, sesame Fusarium wilt is a soil-borne disease, and the effects of agricultural control and chemical control Not obvious enough
So far, there is no good control technology and method for sesame wilt at home and abroad.

Method used

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  • Biocontrol bacteria, separation method, bacterial agent and application thereof for preventing and treating sesame fusarium wilt
  • Biocontrol bacteria, separation method, bacterial agent and application thereof for preventing and treating sesame fusarium wilt
  • Biocontrol bacteria, separation method, bacterial agent and application thereof for preventing and treating sesame fusarium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the isolation of pathogenic bacteria

[0041] Use a sterile cutting knife to cut the stem into 3cm small pieces at the junction of sesame stem disease, sterilize the surface with 75% alcohol for 30 seconds, disinfect the surface with 3% sodium hypochlorite for 5 minutes, and then rinse it with sterile water for 4 times; then use a sterile cutting knife Cut the small section of the stem longitudinally in half from the middle to expose the vascular tissue; gently forceps the central vascular tissue and put it on the PDA medium containing 50 μg / mL chloramphenicol. After 3 days of constant temperature and moisture cultivation in a 26-28°C incubator, the dominant colony PDA was selected and cultured for 3 days, and the single spores were isolated and purified, and stored for future use.

Embodiment 2

[0042] Embodiment 2, the separation of soil microorganism

[0043] Take 1g of soil from the roots of Snakegrass collected, and dilute it to 10 with sterile water -5 -10 -3 g / mL, spread the diluted mixture evenly on a 1 / 10 TSA culture plate, and incubate it upside down in a constant temperature incubator at 28°C for 2 days. Bacteria with different shapes were separated and purified by streaking on the surface of TSA medium.

Embodiment 3

[0044] Embodiment 3, biocontrol bacteria screening

[0045] The plate confrontation culture method was used for the screening of bio-control bacteria. The Fusarium oxysporum block was placed in the center of the PDK plate, and the test bacteria were received at a distance of 25mm from the block, and placed in a 26-28°C incubator for 6 days to observe whether there was an inhibition zone. And measure the size of the inhibition zone, such as figure 1 shown.

[0046] The results of antagonism tests showed that a biocontrol fungus against Sesame wilt was found in the root soil of Snakegrass, named SF3-33.

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Abstract

The invention discloses a bio-control bacterium for preventing and treating sesame wilt disease, a separation method, an inoculant prepared from the bio-control bacterium, and an application of the inoculant. The bio-control bacterium is bacillus licheniformis SF3-33; the preservation organization is china center for typical culture collection (CCTCC); the address of the preservation organization is preservation center of Wuhan University, Luojia Mountain road, Wuchang district, Wuhan city, Hubei province; the preservation date is November 28th, 2012; and the preservation number is CCTCC No. M20122488. The bio-control bacterium is prepared by the following steps: carrying out a microorganism separation treatment and a confront culture treatment on soil of hispid arthraxon roots, and then carrying out a separation treatment so as to obtain the bio-control bacterium for preventing and treating the sesame wilt disease. The bio-control bacterium obtained through a separation treatment can effectively prevent and treat the sesame wilt disease and is capable of promoting the growth of sesame at the same time. The inoculant prepared from the bio-control bacterium can be used to irrigate the sesame roots, the severity degree of the sesame wilt disease can be reduced to 10% or less, the prevention effect can reach 80% or more, and the inoculant also has a prominent growth-promoting effect on sesame plants.

Description

technical field [0001] The invention relates to a biocontrol bacterium for preventing and treating sesame wilt, and at the same time relates to a method for separating the biocontrol bacterium, the biocontrol bacterium agent and its application, and belongs to the technical field of plant protection. Background technique [0002] Sesamum (Sesamum indicum), belonging to the family Flaxaceae, is one of the oldest oil crops in the world and an important high-quality oil crop in my country. The annual planting area of ​​sesame in my country is about 12 million mu, and the total output is about 750,000 tons, which plays an important role in the international oil trade. Sesame Fusarium wilt (SFW) is a soil-borne fungal disease caused by the infection of Fusarium oxysporum f.sp.sesami (Zaprometoff) Castellani (FOS). Sesame can be infected by Fusarium oxysporum during the whole growth period. The disease is a worldwide disease, and many sesame-producing countries such as India, Su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/10
Inventor 张海洋苗红梅常淑娴段迎辉魏利斌
Owner HENAN ACAD OF AGRI SCI
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