Method for preparing microbial inoculum and in-situ decomposing method and structure by heaping whole corn stalks
A technology for the preparation of corn stalks and strains, which is applied in the preparation of organic fertilizers, organic fertilizers, and biological organic parts treatment, etc. difficult effect
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Embodiment 1
[0048] Embodiment 1, the first choice of the present application relates to the preparation method of decomposing bacteria agent, which comprises the following steps
[0049] A. Strain screening
[0050] Take 1000g of fresh livestock feces, add 4000mL sterile water, stir for 5min, centrifuge for 10min, and take the supernatant for later use;
[0051] B. Preparation of decomposing bacteria agent
[0052] (1) Preparation of the original strain: Take 1000g of crushed filter paper, 80g of Na2HPO4, 50g of KH2PO4, 75g of peptone and 30L of water with a pH of 6.5~7.5 in a fermenter, sterilize at 121°C for 30min, cool to 55°C±0.5°C, add A The supernatant in the step is 3500mL, kept at 55°C±0.5°C, stirred and cultured at 60 rpm for 120 hours, and the original seed of the fermentation broth is obtained;
[0053] (2) Preparation of mother seed: Take rice bran, crop straw crushed to less than 3mm, water, urea and fermentation liquid original seed, mix evenly according to the mass ratio ...
Embodiment 2
[0058] Embodiment 2, the first choice of the present application relates to the preparation method of decomposing bacteria agent, which comprises the following steps
[0059] A. Strain screening
[0060] Take 1000g of fresh livestock feces, add 4000mL sterile water, stir for 5min, centrifuge for 10min, and take the supernatant for later use;
[0061] B. Preparation of decomposing bacteria agent
[0062] (1) Preparation of the original strain: Take 1000g of crushed filter paper, 80g of Na2HPO4, 50g of KH2PO4, 75g of peptone and 30L of water with a pH of 6.5~7.5 in a fermenter, sterilize at 121°C for 30min, cool to 55°C±0.5°C, add A The supernatant in the step is 3500mL, kept at 55°C±0.5°C, stirred and cultured at 60 rpm for 120 hours, and the original seed of the fermentation broth is obtained;
[0063] (2) Preparation of mother seed: Take rice bran, crop straw crushed to less than 3mm, water, urea and fermentation liquid original seed, mix evenly according to the mass ratio ...
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