Preparation method of composite microbial starter culture and application thereof
A technology of compound microorganisms and starter, applied in the field of food processing, can solve the problems of unguaranteed product safety, long fermentation production cycle, easy changes in the activity and proportion of fermentation strains, etc., to achieve production controllability and modern production. , the effect of improving quality
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Embodiment 1
[0015] 1.1 Preparation of medium
[0016] The culture medium of Lactobacillus plantarum and Lactobacillus sake is MRS medium, and the specific components are: 10g peptone, 10g beef extract, 5g yeast extract, 2g diammonium hydrogen citrate, 20g glucose, Tween 80 1mL, sodium acetate 5g, phosphoric acid Dipotassium hydrogen 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL, pH value 6.2~6.6, steam sterilization at 121℃ for 15min.
[0017] Lactococcus lactis and Leuconostoc enterococci culture medium M17 medium, the specific components are: tryptone 5g, fish peptone 5g, beef extract 5g, yeast extract 2.5g, ascorbic acid 0.5g, magnesium sulfate 0.25g, glycerol 10.0g , lactose 3g, dipotassium hydrogen phosphate 5g, distilled water 1000mL, plus 1% glucose. Adjust the pH value to 6.7~7.1 with NaOH, and steam sterilize at 121°C for 15 minutes.
[0018] The culture medium of Hansenula debari is PDA medium, and the specific ingredients are: 200g of potatoes, ...
Embodiment 2
[0042] Prepare freeze-dried starter according to the method in embodiment 1, compound as follows:
[0043] Mix Lactobacillus plantarum, Lactobacillus sake, Lactococcus lactis, Leuconostoc enterococci and Hansenula debari according to the mass ratio of 2:2:1:1:1 to prepare a compound microbial starter .
[0044] The application of composite starter in the preparation of sour cabbage, its concrete steps are as follows:
[0045] Wash the selected fresh, disease-free and mildewed cabbage with clean water, place them layer by layer in the fermentation container to 90% of the volume of the container, and then add sterilized water cooled to 20~25°C (boiling at 100°C for 30 minutes) ) to the top of the cabbage just covered; according to the inoculation amount of 0.15‰~0.5‰ of the cabbage weight, add the compound microbial starter, and add the Ca with a final concentration of 0.4~0.6‰ 2+ ; Sealed fermentation at 20-25°C for 72-120 hours to obtain the finished product of fermented sour ...
Embodiment 3
[0047] Prepare freeze-dried starter according to the method in embodiment 1, compound as follows:
[0048] Mix Lactobacillus plantarum, Lactobacillus sake, Lactococcus lactis, Leuconostoc enterococci and Hansenula debari according to the mass ratio of 3:3:2:2:2 to prepare a compound microbial starter .
[0049] The application of composite starter in the preparation of sour cabbage, its concrete steps are as follows:
[0050] Wash the selected fresh, disease-free and mildewed cabbage with clean water, place them layer by layer in the fermentation container to 90% of the volume of the container, and then add sterilized water cooled to 20~25°C (boiling at 100°C for 10 minutes) ) to the top of the cabbage just covered; according to the inoculation amount of 0.15‰~0.5‰ of the cabbage weight, add the compound microbial starter, and add the Ca with a final concentration of 0.4~0.6‰ 2+ ; Sealed fermentation at 20-25°C for 72-120 hours to obtain the finished product of fermented sou...
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