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Chemical sensor for dopamine detection, chemical sensor preparation method, dopamine detection method and application of chemical sensor

A sensor and dopamine technology, applied in the field of chemical sensors, can solve the problems of high equipment requirements, poor selectivity, and low sensitivity, and achieve the effects of high detection sensitivity, easy operation, and obvious selectivity

Inactive Publication Date: 2014-06-04
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the purpose of the present invention is to overcome the defects of poor selectivity, complicated operation, low sensitivity and high equipment requirements in the existing dopamine detection method, and provide a kind of method with good selectivity, high sensitivity and no need to rely on large complex instruments. Chemical sensor for detecting dopamine with convenient operation, preparation method thereof, method for detecting dopamine, and application of the chemical sensor in preparing a device for detecting dopamine

Method used

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  • Chemical sensor for dopamine detection, chemical sensor preparation method, dopamine detection method and application of chemical sensor
  • Chemical sensor for dopamine detection, chemical sensor preparation method, dopamine detection method and application of chemical sensor
  • Chemical sensor for dopamine detection, chemical sensor preparation method, dopamine detection method and application of chemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] This example is used to illustrate the preparation of gold-containing nanoparticles.

[0061] All glassware is pre-washed and oven-dried before use using aqua regia and deionized water. In a 250ml round bottom flask, heat 100ml of 1.0mM tetrachloroauric acid (HAuCl 4 4H 2 O) When the aqueous solution boils, add 10ml of 38.8mM sodium citrate solution, and continue heating and boiling for 30 minutes. The solution color changed from light yellow to wine red during this time, indicating the formation of gold nanoparticles. Then the solution was cooled, centrifuged and washed with deionized water several times, and filtered with a filter membrane with a pore size of 0.2 μm to obtain a gold nanoparticle sol.

[0062] Determination of the absorption spectrum of the prepared gold nanoparticle sol, such as figure 1 As shown, the calculated concentration of gold nanoparticles is about 11.5nM. The gold nanoparticle sol has the maximum absorption at the wavelength of 521nm, an...

Embodiment 2

[0064] This example is used to illustrate the chemical sensor of the present invention and its preparation.

[0065] Add 2ml of 1mM pyridine-4-boronic acid (PDBA) and 0.5mM of 3,3'-dithiodipropionic acid bis(N-hydroxybutanedi Imide ester) (DSP) solution, stirred for about 2 hours to prepare a chemical sensor, as shown in the following formula:

[0066]

[0067] A total of 20 dopamine solutions were prepared with concentrations of 2 μM, 8 μM, 12 μM, 16 μM, 20 μM, 24 μM, 28 μM, 32 μM, 36 μM, 40 μM, 44 μM, 48 μM, 52 μM, 56 μM, 60 μM, 64 μM, 68 μM, 72 μM, 76 μM, and 80 μM, And mix 5 μl of dopamine solution of different concentrations with 3ml of chemical sensor, the dopamine concentration range after the solution is mixed is 3.3nM~133.3nM, so that different color changes occur in the chemical sensor, specifically as image 3 shown.

[0068] The aggregation of gold nanoparticles under the concentration of 33.3nM and 100nM dopamine was observed by SEM as follows: Figure 4 wit...

Embodiment 3

[0073] This example is used to illustrate the chemical sensor of the present invention and its preparation.

[0074] Add 2ml of 1mM PDBA and 0.01mM solution of 4-mercaptobenzo18-crown-6 (ABCE) to the 100ml gold nanoparticle sol prepared in Example 1 at room temperature, and stir for about 2 hours to obtain chemical sensor, as follows:

[0075]

[0076] According to the same method as Example 2, prepare different concentrations of dopamine aqueous solution, add 5 μ l of different concentrations of dopamine aqueous solution to the chemical sensor of 3ml, so that the dopamine concentration is in a linear gradient within 3.3 ~ 133.3nM, in the process can be The color of the chemosensors was observed to change (essentially from wine red to dark blue) with the addition of dopamine, and the color varied according to the amount of dopamine added, as shown in Figure 9 As shown, it shows that the gold nanoparticles in the chemical sensor have aggregated.

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Abstract

The present invention provides a chemical sensor for dopamine detection. The chemical sensor comprises gold-containing nanoparticles, wherein the surface of the gold-containing nanoparticles is modified by class A organic function small molecules and class B organic function small molecules, the class A organic function small molecules contain a boric acid group capable of acting with o-diphenol hydroxyl in the dopamine molecule, the class B organic function small molecules contain one or a plurality of materials selected from a crown ether group, an aldehyde group, succimide activated carboxyl, a phosphate ion group and a sulfonate ion group and capable of acting with the protonated terminal amino group in the dopamine molecule, and the class A organic function small molecules and the class B organic function small molecules further contain one or a plurality of materials selected from pyridine, mercapto, a primary amine group and a disulfide group so as to modify the gold-containing nanoparticles. The invention further provides a preparation method for the chemical sensor, a method for detecting dopamine, and an application of the chemical sensor in dopamine detection apparatuses.

Description

technical field [0001] The invention relates to a chemical sensor, in particular to a chemical sensor capable of detecting dopamine and a preparation method thereof, a method for detecting dopamine and the application of the chemical sensor in preparing a device for detecting dopamine. Background technique [0002] Dopamine (Dopamine, C 6 h 3 (OH) 2 -CH 2 -CH 2 -NH 2 ), referred to as DA, is the precursor of norepinephrine, an important neurotransmitter used by brain endocrine to help cells transmit pulses, and plays an important role in many biopharmacological and physiological processes, and some diseases Production is also closely related to changes in the concentration of dopamine. Insufficient or imbalanced dopamine will cause people to lose the ability to control muscles. In severe cases, it will cause the patient's hands and feet to vibrate involuntarily or lead to Parkinson's disease, ADHD, Huntington's disease and other diseases. In addition, dopamine is an a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 张雪华张鑫西瓦库玛·帕拉尼萨梅贺涛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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