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Honeysuckle contrasting extract and preparation method thereof

A technology of honeysuckle and extract, which is applied in the field of Chinese medicine extract and quality control of Chinese medicine, can solve the problems of incomplete testing standards and high testing costs, and achieve the effects of high detection efficiency, low separation cost, and low cost

Active Publication Date: 2014-05-28
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of incomplete detection standards in the detection of honeysuckle extract and its pharmaceutical preparations in the prior art and the high cost of using multiple reference substances, the present invention provides a method that can simultaneously detect honeysuckle extract and its pharmaceutical preparations. The honeysuckle control extract for the detection of 6 caffeoylquinic acid components in the preparation can be used for the quality control of traditional Chinese medicines containing honeysuckle in the prescription, and the above 6 components can be accurately qualitatively and quantitatively determined

Method used

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  • Honeysuckle contrasting extract and preparation method thereof
  • Honeysuckle contrasting extract and preparation method thereof
  • Honeysuckle contrasting extract and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Take 10.5g of honeysuckle, add water to decoct three times, add 100ml water to decoct for 1 hour for the first and second time, add 50ml water to decoct for 1 hour for the third time, filter the decoction, combine the filtrates, concentrate under reduced pressure to 70 The relative density at ℃ is 1.15, about 15ml; then add 20ml of ethanol, stir evenly, let it stand for 12 hours, take the supernatant to recover ethanol under reduced pressure, and dry to obtain 2.1g of crude extract of honeysuckle. The total content of the six target components in the crude extract was about 8.9% as determined by the HPLC external standard method. The liquid chromatogram of the crude extract is shown in the appendix figure 1 , the contents of the 6 caffeoylquinic acid components are shown in the following table 1:

[0038] Table 1 Contents of 6 target components in the crude extract of honeysuckle

[0039]

[0040] (2) Take the crude extract of honeysuckle prepared in step (1), ...

Embodiment 2

[0049] (1) Take 350g of honeysuckle, add water to decoct three times, add 3L water to decoct for 1 hour for the first and second time, add 2L water for 1 hour to decoct for the third time, filter the decoction, combine the filtrates, and concentrate under reduced pressure to 70°C The relative density is 1.14, about 500ml; then add about 700ml of ethanol, stir evenly, let it stand for 12 hours, take the supernatant to recover ethanol under reduced pressure, and dry to obtain 81g of honeysuckle crude extract.

[0050] The total content of the six target components in the crude extract was about 8.4% as determined by the HPLC external standard method. The liquid chromatogram of the crude extract is shown in the appendix image 3 , the contents of the 6 caffeoylquinic acid components are shown in the following table 3:

[0051] Table 3 Contents of 6 target components in the crude extract of honeysuckle

[0052]

[0053] (2) Take the crude extract obtained in step (1), add 400...

Embodiment 3

[0060] Take Yinhuang tablets with batch number 090901h from a pharmaceutical manufacturer in Hainan as a sample, and prepare the test solution according to the method specified in the second supplement of "Chinese Pharmacopoeia" 2010 edition Yinhuang tablets [Assay]. The control solution was prepared respectively with the honeysuckle control extract obtained in Examples 1 and 2 and a single reference substance (the source of the reference substance: chlorogenic acid, provided by the China Institute for Food and Drug Control, batch number 110753-201314, neochlorogenic acid, cryptochlorogenic Acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C were purchased from Chengdu Pusi Biotechnology Co., Ltd., the batch numbers are ps100603-07, ps100512-06, ps08103101, ps08103102, ps100623-03.) , the content of 6 caffeoylquinic acid components in the sample was determined by external standard method, the results are shown in the following table 5:

[0061] Table ...

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Abstract

The invention relates to the technical field of traditional Chinese medicine extracts and traditional Chinese medicine quality control, in particular to a honeysuckle contrasting extract. The honeysuckle contrasting extract is prepared from the following components of neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C at the mass ratio: (0.20-0.40) : 1.00 : (0.30-0.50) : (0.15-0.45) : (0.10-0.40) : (0.15-0.45), and the total mass fraction exceeds 70%. The preparation method comprises the following steps: performing water extracting and alcohol precipitating to obtain crude extracts; dissolving the crude extracts, regulating pH to be acidic, and centrifuging; separating the supernatant by macroporous adsorption resin, silica gel column and gel column to reach the target content. The honeysuckle contrasting extract has high content of target components, can be applied as a mixed reference substance, is used for quality control of traditional Chinese medicine that contains honeysuckle in the prescription and for accurately qualitative and quantitative measurement, and is efficient in detection, low in cost, accurate in results and lower in preparation cost.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extracts and quality control of traditional Chinese medicines, in particular to a control extract of honeysuckle and a preparation method of the control extract of honeysuckle. Background technique [0002] In the current standard of traditional Chinese medicine, the quality control mode of a single component is difficult to reflect the quality of traditional Chinese medicine, and the method of comprehensive quality control using multiple components or characteristic chromatographic peak groups has attracted more and more attention. However, the shortage of commercial reference substances and the high cost of detection limit the application of multi-component quality control in actual production, scientific research and regulatory fields. The use of control extracts can not only solve the problem of difficult and scarce preparation of monomer reference substances, but also ach...

Claims

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Application Information

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IPC IPC(8): G01N33/15
Inventor 郭东晓林永强徐丽华林林尹宁宁汪冰
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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