Series quadrupole-rod gas-chromatographic mass spectrometry detection method for 35 toxic medicaments in urine

Abstract

The invention discloses a series quadrupole-rod gas-chromatographic mass spectrometry detection method for 35 toxic medicaments in urine. The series quadrupole-rod gas-chromatographic mass spectrometry detection method comprises the steps of constructing a multiple-reaction detection method on a series quadrupole-rod gas chromatograph-mass spectrometer through standard substances of 35 toxicants (medicines), and determining 2-3 pairs of feature parent ions and daughter ions and the retention time of each toxicants (medicines); extracting a urine sample to be detected through diethyl ether, performing ultrasonic and centrifugal treatment, blow-drying and dissolving, and then qualitatively and quantitatively analyzing the toxicants (medicines) through a tandem mass spectrum MRM. By the adoption of a series quadrupole-rod gas-chromatographic-mass spectrometry / mass spectrometry method (GC-MS / MS), the method disclosed by the invention can quickly screen 35 conventional toxicants (medicines) in the urine of a human body; a detection result is accurate, sensitive and quick; the recycling rate of toxicants (medicines) is 80.82-118.56 percent; the detection limit is up to 0.0014-1.8 micrograms per microliter.

Description

technical field [0001] The invention belongs to a tandem quadrupole gas chromatography mass spectrometry detection method for 35 kinds of poisons in urine. Background technique [0002] Drug abuse and drug trafficking have become a global social hazard. Once a person is addicted to drugs, he will often indulge in the deformed "pleasure" imagined by drugs, and have no intention of working and studying. Drug use can also cause a series of family and social problems. In view of the serious harm of drug use to families, especially to society, some foreign countries, in addition to taking corresponding management measures for their own drug users, also require applicants to provide proof of non-drug use, such as Russia, Belgium, Hong Kong and so on. As the process of global economic integration accelerates, it is inevitable that other countries will introduce similar regulations for immigrants one after another. In order to actively respond to the requirements of some foreign ...

AI Technical Summary

Problems solved by technology

[0010] In the comparison document with Chinese patent number 201110075177.4, the urine is pretreated by the classic liquid-liquid extraction method, ethyl acetate is used as the extraction solvent, and the total amount of extraction agent is 20ml, and only 33 kinds of drugs can be detected

Therefore, the detection should be divided into two detections, which cannot achieve true one-time detection.

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