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A filter-membrane-based forensic diatom detection method

An inspection method and forensic technology, which are applied in scientific instruments, preparation of test samples, material analysis by optical means, etc., can solve the problems that cannot be widely used in grassroots forensic laboratories, cannot significantly improve the recovery rate of diatoms, and increase the centrifugal speed. and other problems, to achieve the effect of clear diatom texture and details, easy promotion and improved recovery rate

Active Publication Date: 2016-02-10
GUANGZHOU CRIMINAL SCI & TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to research reports by Hu Sunlin et al., one centrifugation results in about 10% loss of diatoms, and increasing the centrifugation speed cannot significantly increase the recovery rate of diatoms, thus resulting in a low recovery rate of diatoms in organ tissues.
[0005] Diatoms can also be enriched by vacuum filtration, but the filter membrane itself is opaque and cannot be directly observed under an optical microscope. Therefore, Hu Sunlin et al. tried to use vacuum filtration-automated scanning electron microscopy to observe diatoms
Although the electron microscope has high resolution and clear observation, due to its complex operation, high cost and high maintenance cost, the electron microscope can only be used in some large and medium-sized laboratories and scientific research institutions, and cannot be widely used in grassroots forensic science laboratory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A filter-based forensic diatom detection method: divided into three steps:

[0024] S1. Membrane enrichment:

[0025] Place 50-100ml of sample (diluted tissue and organ digestion solution) in the suction cup of a single or multiple vacuum filtration device to start suction filtration; vacuum degree 100-800mmHg, polyethersulfone (PES) filter membrane diameter 10-50mm, the pore diameter is 0.1-1.0μm. When the suction filtration of the sample is completed, add 50-200ml of ultra-pure water to continue the suction filtration, so that the surface of the filter membrane is close to neutral.

[0026] S2. Membrane transparency:

[0027] After the suction filtration is completed, place the filter membrane on an electric furnace at 50-80°C or heat it in an oven for 1-10 minutes to make it completely dry; take an unused glass slide, and add 1-5 drops of acetic acid-eugenol reagent dropwise in the center; acetic acid - The eugenol reagent is a mixed solution obtained by uniformly...

Embodiment 2

[0031] A filter-based forensic diatom detection method: divided into three steps:

[0032] S1. Membrane enrichment:

[0033] Place 50-100ml of sample (diluted tissue and organ digestion solution) in the suction cup of a single or multiple vacuum filtration device to start suction filtration; vacuum degree 100-800mmHg, polyethersulfone (PES) filter membrane diameter 10-50mm, the pore diameter is 0.1-1.0μm. When the suction filtration of the sample is completed, add 50-200ml of ultra-pure water to continue the suction filtration, so that the surface of the filter membrane is close to neutral.

[0034] S2. Membrane transparency:

[0035] After the suction filtration is completed, place the filter membrane on an electric furnace at 50-80°C or heat it in an oven for 1-10 minutes to make it completely dry; take an unused glass slide, and add 1-5 drops of acetic acid-eugenol reagent dropwise in the center; acetic acid - The eugenol reagent is a mixed solution obtained by uniformly...

Embodiment 3

[0039] A filter-based forensic diatom detection method: divided into three steps:

[0040] S1. Membrane enrichment:

[0041]Place 50-100ml of sample (diluted tissue and organ digestion solution) in the suction cup of a single or multiple vacuum filtration device to start suction filtration; vacuum degree 100-800mmHg, polyethersulfone (PES) filter membrane diameter 10-50mm, the pore diameter is 0.1-1.0μm. When the suction filtration of the sample is completed, add 50-200ml of ultra-pure water to continue the suction filtration, so that the surface of the filter membrane is close to neutral.

[0042] S2. Membrane transparency:

[0043] After the suction filtration is completed, place the filter membrane on an electric furnace at 50-80°C or heat it in an oven for 1-10 minutes to make it completely dry; take an unused glass slide, and add 1-5 drops of acetic acid-eugenol reagent dropwise in the center; acetic acid - The eugenol reagent is a mixed solution obtained by uniformly ...

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Abstract

The invention relates to the field of algae inspection, and specifically discloses a forensic diatom inspection method based on a filter membrane. In the present invention, after the vacuum-filtered filter membrane is transparentized, the transparent filter membrane can be directly observed under an optical microscope. The detection method of the invention is easy to operate, high in detection efficiency, does not need to rely on complex instruments, and can be detected in basic forensic laboratories, and has wider applicability.

Description

technical field [0001] The invention relates to the field of algae inspection, in particular to a forensic diatom inspection method based on a filter membrane. Background technique [0002] Diatom is a kind of single-celled algae. There are more than 16,000 kinds of diatoms in the world. The body length is generally between 1 μm and 200 μm. A transparent shell with shell rings fit together to form a siliceous cell wall. The living environment of diatoms is very wide, and diatoms can be found in almost all waters in nature. [0003] Dead bodies in water are a common type of forensic autopsy. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter multiple organs from the circulatory system. The diatom cell wall is not easy to be destroyed because of its strong resistance, and those with high silicon content will not be destroyed by boiling with concentrated sulfuric acid or concentrated nitric acid or even burning at hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/28
Inventor 刘超胡孙林温锦锋赵建胡韧黎乾石河王玉仲
Owner GUANGZHOU CRIMINAL SCI & TECH RES INST
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