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Method of extracting polysaccharide of enteromorpha by enzymolysis in steps

A step-by-step enzymatic hydrolysis technology of Enteromorpha polysaccharides, which is applied in the direction of anti-toxic agents and drug combinations, can solve the problems of low activity, high energy consumption, and low yield of polysaccharides, and achieve high biological activity, improved extraction efficiency and purity, Effects that are beneficial to development and utilization

Inactive Publication Date: 2014-05-21
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of polysaccharides extracted from Enteromorpha by hot water extraction is low, the impurity content is high, the energy consumption is high, and the activity is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Clean the enteromorpha, dry it at 60°C, crush it, and pass it through a 40-mesh sieve. Weigh 1kg of Enteromorpha powder, add 20L of distilled water, stir well, soak for 15h. Get the first extract of Enteromorpha.

[0020] Step 2: Add citric acid to the first extract of Enteromorpha to adjust the pH value to 4.0, heat the temperature to 45°C, add 15g of pectinase, stir well, and enzymatically hydrolyze for 1 hour to obtain the second extract of Enteromorpha .

[0021] Step 3: Add 0.1M sodium hydroxide to the second extract of Enteromorpha to adjust the pH value to 5.0, heat the temperature to 55°C, add 10g of cellulase, stir well, and enzymatically hydrolyze for 2 hours to obtain the third Enteromorpha Extraction solution.

[0022] Step 4: Add 30 g of papain to the third extract of Enteromorpha, adjust the pH value to 5.5 with 0.1 M sodium hydroxide, and perform enzymatic hydrolysis at a temperature of 55° C. for 2 hours to obtain the fourth extract of Enterom...

Embodiment 2

[0026] Step 1: Clean the enteromorpha, dry it at 60°C, crush it, and pass it through a 40-mesh sieve. Weigh 1kg of Enteromorpha powder, add 20L of distilled water, stir well, soak for 10h. Get the first extract of Enteromorpha.

[0027] Step 2: Add citric acid to the first extract of Enteromorpha to adjust the pH to 4.0, heat the temperature to 40°C, add 10 g of pectinase, stir well, and enzymatically hydrolyze for 1 hour to obtain the second extract of Enteromorpha .

[0028] Step 3: Add 0.1M sodium hydroxide to the second extract of Enteromorpha to adjust the pH to 5.0, heat the temperature to 50°C, add 8g of cellulase, stir well, and enzymatically hydrolyze for 2 hours to obtain the third Enteromorpha Extraction solution.

[0029] Step 4: Add 30 g of papain to the third extract of Enteromorpha, adjust the pH value to 5.5 with 0.1 M sodium hydroxide, and perform enzymatic hydrolysis at a temperature of 55° C. for 2 hours to obtain the fourth extract of Enteromorpha.

[0...

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Abstract

The invention provides a method of extracting polysaccharide of enteromorpha by enzymolysis in steps. The method comprises the following steps: by taking enteromorpha as a raw material, soaking the enteromorpha powder by water after impurity removal and crushing; then, respectively carrying out enzymolysis in steps on the aqueous extract of the enteromorpha by using pectinase, cellulase and papain; then, inactivating the enzyme, concentrating by centrifuging and precipitating by ethanol; and finally, vacuum-drying to obtain the polysaccharide of the enteromorpha. The method provided by the invention is high in yield, high in product purity, low in energy consumption, free from pollution, high in biological activity, suitable for production of polysaccharide of enteromorpha on a large scale, beneficial to development and utilization of resources polysaccharide of enteromorpha, and remarkable in economic and social values.

Description

technical field [0001] The invention relates to a method for extracting Enteromorpha polysaccharide, in particular to a step-by-step enzymolysis method for extracting Enteromorpha polysaccharide. Background technique [0002] Enteromorpha (Enteromorpha) is a large green algae, commonly known as moss strips, green seaweed, etc., which is an algae plant of the genus Enteromorpha in the order Ulvaceae of the Chlorophyta Ulvaidae, which grows in the intertidal zone. In China, the production of Enteromorpha is very high, especially along the eastern coast. In recent years, the rapid propagation of Enteromorpha has caused pollution to the coastal areas of my country. To control the pollution caused by the excessive propagation of Enteromorpha, it takes a lot of manpower and material resources. Therefore, if Enteromorpha can be developed and utilized, it will not only control the pollution of Enteromorpha, but also Can turn waste into treasure. [0003] Enteromorpha has been edibl...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
Inventor 唐志红鞠宝任育红吕家森温少红
Owner YANTAI UNIV
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