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Method for detecting urotropine in cigarette side stream smoke through high performance liquid chromatography-tandem mass spectrometry

A technology of high-performance liquid chromatography and sidestream smoke, which is applied in the field of cigarette smoke, can solve the problems of complex composition of sidestream smoke, no detection of cigarette urotropine, matrix interference, etc., and achieves a simple, environmentally friendly, Measuring precise effects

Active Publication Date: 2014-05-14
CHINA TOBACCO JIANGSU INDAL
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As the public pays more and more attention to smoking and health problems, the harmful component urotropine in cigarette smoke has become the focus of people's attention. Due to the complex composition of sidestream smoke, various components, and serious matrix interference, there is currently no There are no literature reports or patents on the detection of urotropine in cigarette sidestream smoke

Method used

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  • Method for detecting urotropine in cigarette side stream smoke through high performance liquid chromatography-tandem mass spectrometry
  • Method for detecting urotropine in cigarette side stream smoke through high performance liquid chromatography-tandem mass spectrometry

Examples

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Effect test

Embodiment 1

[0019] A method for detecting urotropine in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrometry, comprising the following steps:

[0020] Smoking cigarette samples: Select a commercially available cigarette A and balance it for 48 hours under the conditions of (22±1)°C and relative humidity (60±3)%, and then follow the "weight tolerance ± 20mg, draw resistance tolerance ± 50Pa" Screen the cigarettes according to the requirements, then mark the smoking stop line of the cigarettes, adjust the suction flow rate of the sidestream smoking machine to 3000mL / min according to the sidestream smoke suction method in YC / T185, smoke 2 cigarettes, and use a The 25mL absorption bottle, fishtail hood and glass fiber filter disc capture the total particulate matter of sidestream smoke.

[0021] After suction, use 1 / 4 of the filter to wipe the trap and combine it with the filter containing sidestream smoke particulate matter, put it into a 100mL Erlenm...

Embodiment 2

[0026] According to the method of Example 1, a certain cigarette was measured in parallel 12 times, and the repeatability results of the obtained samples are shown in Table 1.

[0027] Table 1 Parallel experiments of a certain cigarette sample (unit: μg / cig)

[0028]

[0029] The results in Table 1 show that the relative standard deviation is less than 5%, indicating that the content of urotropine in cigarette sidestream smoke can be accurately determined.

[0030] The standard sample with the lowest concentration (10ng / L) was injected 10 times, and the standard deviation was calculated from the measured concentration value. The limit of quantification was obtained by 10 times the standard deviation. The limit of quantification of this method is 1.83 μg / cig, indicating that The sensitivity of the method is high.

[0031] Adding 20.45 μg, 50.78 μg and 89.37 μg of urotropine standard substances to a sidestream Cambridge filter of a cigarette, the release amount of urotropine...

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Abstract

The invention discloses a method for detecting urotropine in cigarette side stream smoke through high performance liquid chromatography-tandem mass spectrometry. The method comprises the following steps: trapping the urotropine in cigarette side stream smoke by utilizing a glass fiber filter disc, extracting the filter disc with acetonitrile, cleaning a fishtail cover and a pipeline, respectively taking 0.5mL of extracting solution and 0.5mL of cleaning solution, merging the solutions, purifying through an organic phase filter membrane, detecting through LC-MS / MS, and analyzing the content of the urotropine in the cigarette side stream smoke. The content of the urotropine in the cigarette side stream smoke is measured by adopting LC-MS / MS, qualitative detection is performed by adopting a MRM (Multi-Reaction Monitoring) mode, the qualitative accuracy and the sensitivity of the method can be effectively improved, and the method is suitable for measuring the urotropine in various cigarette side stream smokes. The application range is wide, the measurement is accurate, and the method is simple and environment-friendly and has high practicality.

Description

technical field [0001] The invention belongs to the technical field of cigarette smoke, and in particular relates to a method for detecting urotropine in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrometry (LC-MS / MS). Background technique [0002] Environmental tobacco smoke (ETS) is an important indoor pollutant, which is what passive smokers feel, and the sidestream smoke of cigarettes is the main source of environmental smoke, accounting for about 85% of environmental smoke. At present, some countries and regions such as Canada have designated relevant laws, requiring cigarette manufacturers to provide the release amount of harmful components in cigarette smoke. In view of this, it is necessary to conduct an in-depth analysis of harmful components in sidestream smoke. Sidestream smoke is the smoke substance released directly from the cigarette burning cone into the air and diffused from the cigarette paper. Most of the sidestream ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 沈晓晨庄亚东张华石怀彬王晨辉曹毅朱怀远韩开冬张媛朱莹何红梅盛金
Owner CHINA TOBACCO JIANGSU INDAL
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