Biological feed for soft-shelled turtles and production process of biological feed
A technology of biological feed and soft-shelled turtle, which is applied in the field of feed, can solve problems such as the decline of the specific immune function of Chinese soft-shelled turtle, the destruction of the ecological balance of the water body, and the threat to human health, so as to avoid drug residues and increased resistance of pathogenic bacteria, maintain long-lasting quality, and be convenient. The effect of storage and transportation
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Embodiment 1
[0031] Embodiment 1: prepare bacillus licheniformis premix
[0032] 1) Inoculate Bacillus licheniformis, and cultivate the production strains on the slant
[0033] The composition of the slant culture medium is: glucose 2%, peptone 0.2%, beef extract 0.5%, cotton cake powder 2%, yeast powder 0.5%, (NH4) 2 HPO 4 0.5%, K 2 HPO 2 0.03%, MgSO 4 ·7H 2 O 0.02%, NaCl 0.5%, MnSO 4 0.003%, agar 1.5%.
[0034] The culture conditions on the slant are: temperature 28~33℃, culture time 24~48h.
[0035] 2) Pick out the well-growing colonies cultured on the slant and carry out shake flask culture;
[0036] The medium for shaking flask culture is: glucose 3%, bean cake powder 1%, cotton cake powder 2%, peptone 0.1%, beef extract 0.5%, yeast powder 0.5%, (NH 4 ) 2 SO 4 0.5%, NaCl 0.5%.
[0037] Shake flask culture conditions are: rotation speed 100~300r / min, temperature 28~33°C, culture time 24~48h.
[0038] 3) Use a 50L seed tank to carry out the first-level liquid seed fermenta...
Embodiment 2
[0055] Example 2 Preparation of biological feed
[0056] Biological feed formula (parts by weight): American marine white fish meal 52.5, α-starch 16.5, puffed soybean 13.2, liver powder 0.18, egg yolk powder 0.038, milk powder 0.02, binder 0.03, lecithin 0.06, multidimensional 0.24, minerals 0.24 , Carnitine 0.02, Betaine 0.024, Bile Salt 0.016, Methionine 0.036, Lysine 0.036, Salt 0.06, Calcium Dihydrogen Phosphate 1.2, Choline Chloride 0.36, Gluten 2, Squid Liver Powder 2, Rice Protein powder 2.6, corn gluten powder 2.22, feed yeast 2.8, Bacillus licheniformis premix 0.12, Chinese herbal medicine preparation 3.5.
[0057] Traditional Chinese medicine formulation (parts by weight): Radix Radix 30, Honeysuckle 1.5, Dandelion 0.15, Licorice 0.1, Rhubarb 0.1, Coptis 4, Scutellaria baicalensis 0.3, Cortex Phellodendron 0.03.
[0058] 1) Mix and grind each Chinese herbal medicine according to the requirements of the formula of Chinese herbal medicine preparation, and mix an...
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