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Algae harvesting method taking modified chitosan as flocculant

A harvesting method and flocculant technology, applied in the field of algae harvesting, can solve the problems of difficulty in collecting microalgae, high energy consumption, low income, etc., and achieve the effect of simple preparation process, moderate cost and low cost

Active Publication Date: 2014-04-30
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims to solve technical problems such as difficulty in collecting existing microalgae, high energy consumption, low yield, high toxicity, etc., and provides a method for collecting algae using modified chitosan as a flocculant. After base modification, it has better flocculation effect, and can be applied to the collection of microalgae after large-scale cultivation and the rapid treatment of harmful algae that lead to eutrophication of water bodies. It has low cost, less investment, and simple preparation process conditions. and environmental friendliness

Method used

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  • Algae harvesting method taking modified chitosan as flocculant
  • Algae harvesting method taking modified chitosan as flocculant
  • Algae harvesting method taking modified chitosan as flocculant

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Add 3 g of chitosan with an average molecular weight of 70 kDa and a degree of deacetylation of 81% to 30 mL of 0.3 mol / L hydrochloric acid solution to completely dissolve the chitosan to obtain a chitosan hydrochloric acid solution.

[0031] Add aminonitrile to the chitosan hydrochloric acid solution, and stir at 90°C for 12 hours to obtain a clear solution, wherein the molar ratio of aminonitrile to chitosan in the chitosan hydrochloric acid solution is 2:1.

[0032] Put the obtained clear solution into a dialysis bag (MWCO3500), dialyze for 5 days, pre-freeze in a low-temperature refrigerator at -20°C, and then dry it in a freeze dryer to obtain a guanidino-chitosan flocculant. The obtained guanidine-chitosan Sugar flocculant (GCS) and former chitosan (CS) Fourier transform infrared spectrum and NMR test results are shown in figure 1 , figure 2 , image 3 .

[0033] figure 1 Middle 1622cm -1 The absorption peak at corresponds to the guanidine group, indicating ...

Embodiment 2

[0036] Add 3 g of chitosan with an average molecular weight of 70 kDa and a degree of deacetylation of 85% to 30 mL of 0.1 mol / L hydrochloric acid solution to completely dissolve the chitosan to obtain a chitosan hydrochloric acid solution.

[0037] Add aminonitrile to the chitosan hydrochloric acid solution, stir at 95°C for 8h to obtain a clear solution, wherein the molar ratio of aminonitrile to chitosan in the chitosan hydrochloric acid solution is 2:1.

[0038] The obtained clear solution was put into a dialysis bag (MWCO2000), dialyzed for 2 days, pre-frozen in a -20°C low-temperature refrigerator, and then dried in a freeze dryer to obtain a guanidino-chitosan flocculant.

[0039] Cultivate Chlorella in the culture medium for 4 days, take 2 mg of the guanidino-chitosan flocculant prepared above and add it to 1L of the microalgae culture medium, so that the concentration of the flocculant is 2 mg / L, stir with a stirrer for 10 min, and culture the microalgae The solution wa...

Embodiment 3

[0041] Add 2 g of chitosan with an average molecular weight of 70 kDa and a degree of deacetylation of 70% to 30 mL of 0.2 mol / L hydrochloric acid solution to completely dissolve the chitosan to obtain a chitosan hydrochloric acid solution.

[0042] Add aminonitrile to the chitosan hydrochloric acid solution, stir at 85°C for 10 hours to obtain a clear solution, wherein the molar ratio of aminonitrile to chitosan in the chitosan hydrochloric acid solution is 3:1.

[0043] The obtained clear solution was put into a dialysis bag (MWCO3500), dialyzed for 5 days, pre-frozen in a -20°C low-temperature refrigerator, and then dried in a freeze dryer to obtain a guanidino-chitosan flocculant.

[0044] Cultivate Chlorella in the culture medium for 3 days, take 50mg of the guanidino-chitosan flocculant prepared above and add it to 1L of the microalgae culture medium to make the concentration of the flocculant 50mg / L, stir with a stirrer for 8min, and culture the microalgae The solution ...

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Abstract

The invention discloses an algae harvesting method taking modified chitosan as a flocculant. The method comprises the following steps: firstly, modifying chitosan to obtain a guanidine chitosan flocculant, and then adding the guanidine chitosan flocculant to a micro algae culture solution, so that the concentration of the guanidine chitosan flocculant in the micro algae culture solution is 2-100mg / L, stirring for 5-10 minutes, standing and participating for 10-120 minutes; removing supernate, and harvesting the micro algae. The chitosan has a better flocculation effect after being modified in a guanidination manner, and can be applied to collection of micro algae after large-scale cultivation, and rapid treatment of a harmful algal body of leading to water eutrophication, and the algae harvesting method has the advantages of low cost, less investment, simple preparation technological condition, environment friendliness and the like.

Description

technical field [0001] The invention belongs to the application field of algae harvesting in the process of preparing biodiesel, in particular relates to a method for algae enrichment and harvesting using modified chitosan as a flocculant. Background technique [0002] my country's oil resources are insufficient, and in recent years it has been heavily dependent on imports, which account for more than half of the total oil. The development of liquid fuels that can replace petroleum resources has become an urgent social problem in today's society, in order to fundamentally solve the current situation of my country's oil shortage and effectively ensure my country's energy security. Therefore, finding alternative fuels for oil, especially diesel oil, has become an urgent and much-needed problem. Biodiesel is a good alternative fuel to fossil diesel. [0003] About 75% of the cost of biodiesel is the cost of raw material oil, so the key to the industrialization of biodiesel li...

Claims

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Application Information

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IPC IPC(8): C12N1/12C08B37/08C12R1/89
Inventor 齐云陈冠益赵刘
Owner TIANJIN UNIV
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