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Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein G

A technology of mutants and outer structures, applied in the field of immunization, can solve the problems of difficult recovery of antibodies and difficult dissociation.

Inactive Publication Date: 2014-04-23
NAT INST OF ADVANCED IND SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once in such a bound state, the antibody and the extracellular domain of protein G are not easily dissociated, making it difficult to recover the antibody

Method used

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  • Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein G
  • Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein G
  • Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein G

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0028] A protein consisting of a tandem multimer of a cell extramembrane domain mutant having binding activity to a protein having an Fc region of immunoglobulin G.

Embodiment approach 2

[0030] The protein according to Embodiment 1, which is a tandem trimer, a tandem tetramer, or a tandem pentamer.

Embodiment approach 3

[0032] The protein according to Embodiment 1 or 2, wherein the extracellular domain mutants constituting the multimer are identical to each other.

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Abstract

The purpose of the present invention is: to provide an excellent protein which is further reduced in the binding property to an Fc region of an immunoglobulin and / or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a protein containing an extracellular domain of wild-type protein G, and which still keeps a high antibody-binding activity in a neutral region; and to capture and collect an antibody readily using the protein without denaturating the antibody. The present invention relates to: a protein that is reduced in the binding property to an Fc region of an immunoglobulin and / or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a multimer comprising an extracellular domain of wild type one, which is a domain having a binding activity to a protein comprising an Fc region of immunoglobulin G, while keeping a high antibody-binding activity in a neutral region, and also has a binding activity to a protein comprising the Fc region of immunoglobulin G, wherein the protein comprises a tandem-type multimer of a mutant of the extracellular domain; and others.

Description

【Technical field】 [0001] The present invention relates to (comprising) a novel modified protein composed of a tandem multimer of mutants of the extracellular domain of antibody-binding protein protein G, a nucleic acid encoding the protein, and an antibody utilizing the antibody-binding property of the protein , a capture agent for immunoglobulin G or a protein having an Fc region of immunoglobulin G, and a chromatographic column for protein separation and purification filled with the capture agent, and the like. 【Background technique】 [0002] Protein G is a protein derived from Streptococcus, a membrane protein present in the cell membrane of Streptococcus genus Streptococcus, and known to specifically bind to the Fc region of Immunoglobulin G (a type of antibody) Activity (Non-Patent Document 1, Patent Document 1). Protein G is a multi-domain membrane protein composed of multiple domains, and a part of it exhibits binding activity to proteins having the Fc region of immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C07K14/315C07K17/00C07K19/00C12N1/15C12N1/19C12N1/21C12N5/10
CPCC07K14/315C07K2319/00C12N9/88C07K1/22C07K2319/70C12N15/62
Inventor 本田真也松丸裕之渡边秀树吉田昼也
Owner NAT INST OF ADVANCED IND SCI & TECH
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