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Pounding and bumping hammer

A technology of bumping hammer and hammer body, which is applied in the field of bumping hammer, which can solve the problems of easy failure of the instrument, failure to meet the severe strength, insufficient vibration force, etc., and achieve the effect of simple operation, obvious homogenization effect and broad application prospects

Active Publication Date: 2014-04-23
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Currently, various reagents, solutions, and chemical substances are fixed, oscillated, For mixing treatment, microbial culture, optimization, screening, and physiological and biochemical reactions of cells and tissues, commonly used shaking devices include: vortex mixer, vortex shaker, constant temperature (full temperature) shaker, constant temperature shaker, micro shaker, Water bath oscillators, etc. These instruments have the following characteristics: (1) need to be driven by electric energy; (2) the speed is 0-3000rpm; (3) the amplitude is less than 35mm (5-35mm); (4) the vibration mode can be divided into vertical , reciprocating, gyrating, vertically reciprocating compound gyrating and reciprocating; (5) The shock force generated is far from enough; (6) The instrument is prone to failure
In molecular biology experiments, researchers often find that these instruments cannot meet the severe intensity required for some special experiments

Method used

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  • Pounding and bumping hammer

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1, smashing hammer of the present invention

[0016] The hammer provided by the present invention (such as figure 1 (shown) is composed of a hammer body and a handle 4 fixedly connected with the hammer body, and the hammer body is composed of a switchable closed box and an elastic component fixedly connected with one end of the closed box. The airtight box is composed of a cover 1 and a cup 2 that cooperate with each other. The elastic component is fixedly connected with the bottom end of the cup body 2 , and the elastic component is a rubber block 3 . The cup body 2 is a cylindrical cavity with transparent side walls. The rubber block is a cylinder with the connection surface with the airtight box as the bottom surface. The height of the airtight box is 10.0cm (8.0-14.0cm is acceptable), and the bottom diameter is 4.0cm (4.0-6.0cm is acceptable). The height of the rubber block is 1.8cm (1.5-2.0cm is acceptable), and the bottom diameter is 4.0cm (4.0-6.0c...

Embodiment 2

[0019] Embodiment 2, the application of the present invention smashing hammer

[0020] 1. Suspension experiment of bacterial sediment

[0021] In the process of molecular biology experiments, engineering bacteria play an irreplaceable role in molecular cloning. Gene cloning and vector construction are mainly divided into steps such as gene amplification, enzyme digestion, connection, transformation and plasmid extraction. Among them, during the plasmid extraction process after the plasmid transforms into engineering bacteria, the resuspension of the bacterial pellet is an important part of extracting the plasmid. The specific steps are as follows (taking Escherichia coli DH5α as an example):

[0022] 1. Acquisition of Bacterial Precipitation

[0023] 1) Plasmid transformed bacteria

[0024] Take 100 μl of thawed competent cells of Escherichia coli (E.coli) DH5a (purchased from Tiangen Biochemical Technology (Beijing, article number: CB101) Co., Ltd.), and add 50 ng of plas...

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Abstract

The invention discloses a pounding and bumping hammer. The pounding and bumping hammer is a vibration apparatus used in molecular biology experiment, and is composed of a hammer body and a handle which is fixedly connected to the hammer body, and the hammer body is composed of an openable and closeable closed box and an elastomeric element which is fixedly connected to one end of the closed box. The closed box is composed of a cover and a cup which are mutually matched. The elastomeric element may be composed of a rubber block. The pounding and bumping hammer provided by the invention is knocked downwards for 5-6 times from the height which is 30 centimeters higher above the desktop, and the bacteria deposition can be suspended totally; the pounding and bumping hammer provided by the invention can be used in homogenization and phase separation of fat sample in fat RNA extraction experiment, and finally the total extracted RNA amount is 10.5 mu g / 100 mg of sample. The pounding and bumping hammer provided by the invention has obvious dispersion effect for objective substances with simple operation and wide application prospect.

Description

technical field [0001] The invention relates to an oscillation device used in molecular biology experiments, in particular to a bump hammer. Background technique [0002] Currently, various reagents, solutions, and chemical substances are fixed, oscillated, For mixing treatment, microbial culture, optimization, screening, and physiological and biochemical reactions of cell tissue, commonly used shaking devices include: vortex mixer, vortex oscillator, constant temperature (full temperature) shaker, constant temperature oscillator, micro shaker, Water bath oscillators, etc. These instruments have the following characteristics: (1) need to be driven by electric energy; (2) the speed is 0-3000rpm; (3) the amplitude is less than 35mm (5-35mm); (4) the vibration mode can be divided into vertical , reciprocating, gyrating, vertically reciprocating compound gyrating and reciprocating; (5) The shock force generated is far from enough; (6) The instrument is prone to failure. In mol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M35/04
Inventor 甘尚权高蕊王新华沈敏
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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