Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of anti-duck viral hepatitis polysaccharide and its molecular modification

A technology of duck viral hepatitis and duck hepatitis virus, which is applied in the direction of antiviral agents, can solve problems such as losses, and achieve the effects of convenient use of preparations, good water solubility, and good water solubility

Active Publication Date: 2016-03-09
NANJING AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once a clinical case occurs, it will cause irreparable losses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of anti-duck viral hepatitis polysaccharide and its molecular modification
  • A kind of anti-duck viral hepatitis polysaccharide and its molecular modification
  • A kind of anti-duck viral hepatitis polysaccharide and its molecular modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Extraction of polysaccharides from bean root and screening of effective fractions

[0017] Weigh 1,000g of the bean root, add 10 times the amount of water to soak for 3 hours, heat and boil for 1 hour, collect the decoction, and repeat 3 times; combine the collected decoction, boil and concentrate to 1000mL, centrifuge with a high-speed centrifuge, and take the supernatant. Slowly add ethanol with different volume fractions of 95% to the concentrated solution so that the ethanol content in the solution is 50%, 65%, 75% and 80% respectively, let it stand overnight, and collect the precipitates by centrifugation respectively. After dialysis with running water, vacuum concentration, 3000rpm / min, and centrifugation for 20min, the supernatant was again added with different volume fractions of 95% ethanol to precipitate, after repeated dialysis, concentration, and centrifugation, vacuum-dried at 60°C, and crushed to obtain different polysaccharides of bean root. Alcohol pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to anti-duck virus hepatitis (DVH) subprostrate sophora polysaccharide and a molecular modifier thereof, belonging to the technical field of preparation of Chinese veterinary medicaments. Different alcohol precipitation fractions of the subprostrate sophora polysaccharide are extracted. An anti-duck virus hepatitis (DVH) active site is screened by means of repeated clinical tests. The following optimal conditions for modifying a subprostrate sophora polysaccharide active site by means of chlorosulfonic acid-pyridine sulfation are determined by using an orthogonal method: the reaction temperature is 95 DEG C, the reaction time is 2 hours, and the ratio of chlorosulfonic acid to pyridine is 1:5. According to infrared spectroscopic analysis, an obtained product is subprostrate sophora polysaccharide sulfate, the saccharide content is up to 83.77 percent, the sulfate content is up to 39.33 percent, and the yield is up to 117.33 percent. The subprostrate sophora polysaccharide sulfate is remarkably superior to the subprostrate sophora polysaccharide in resistance of DHV-1 infection of duck fetal liver cells and curative effect on DVH. The obtained anti-DVH subprostrate sophora polysaccharide active site sulfate is high in saccharide and phosphate contents and yield, and has a good curative effect on DVH.

Description

1. Technical field [0001] The invention relates to an anti-duck viral hepatitis polysaccharide and its molecular modification, and belongs to the technical field of Chinese veterinary medicine preparation. 2. Background technology [0002] Duck viral hepatitis is a disease caused by duck hepatitis virus (DHV) infection that spreads rapidly, has a high morbidity rate, and is highly fatal. The virus was first isolated in 1949 and is now distributed worldwide. DHV mainly has three serotypes: DHV-1 and its variants, DuckAstrovirus and DHV-3, among which DHV-1 is the most toxic and widely distributed, mainly infecting ducklings under 3 weeks old, with a fatality rate higher than 80% %, even up to 100%, is one of the main pathogens that seriously endanger the duck industry. [0003] At present, there is no effective anti-DHV-1 drug clinically in the world, and the immune defense is mainly carried out by injecting attenuated vaccines to breeding ducks or ducklings. Once a clinic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/14
Inventor 刘家国陈云熊文曾玲徐美云王德云胡元亮武毅黄兵马秀丽
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com