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Plant water treatment method of municipal wastewater at high altitude

A technology of urban domestic sewage and treatment method, applied in the field of water treatment of domestic sewage, can solve the problems of low oxygen content, freezing to death, difficult to repair, etc., and achieve the effect of enhancing oxygen binding capacity, improving cold resistance, and improving water treatment effect.

Inactive Publication Date: 2014-04-23
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low oxygen content in water bodies seriously polluted by domestic pollution has become a bottleneck restricting the plant water treatment of Echinochloa chinensis.
Moreover, in high latitudes, although the submerged part of Alpinia sinensis is still active in winter, it can still germinate and grow in the next spring, but the water surface has been frozen to death. Due to the long winter in high latitudes, it is difficult to use it for water restoration in high latitudes.

Method used

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  • Plant water treatment method of municipal wastewater at high altitude
  • Plant water treatment method of municipal wastewater at high altitude
  • Plant water treatment method of municipal wastewater at high altitude

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specific Embodiment approach 1

[0013] Specific implementation mode 1: The plant water treatment method of high-latitude urban domestic sewage in this implementation mode is carried out according to the following steps:

[0014] 1. Construction of plant expression vectors;

[0015] 2. Constructing transgenic plants of A. japonicus;

[0016] 3. Cultivate and propagate transgenic plants of A. japonicus, and then transplant the transgenic plants of A. japonicus to high-latitude polluted water bodies for sewage treatment and restoration;

[0017] Wherein, in the first step, the plant expression vector contains a promoter superpromoter and a hyaline hemoglobin gene.

[0018] In this embodiment, the promoter superpromoter whose nucleotide sequence is shown in SEQ ID NO: 1 is first introduced into the vector p3300-Tnos, and then the synthetic hemoglobin gene (shown in SEQ ID NO: 2) and the promoter superpromoter are introduced into the vector The superpromoter vector p3300-Tnos was double digested with XbaI and S...

specific Embodiment approach 2

[0021] Specific embodiment two: the difference between this embodiment and specific embodiment one is: after the transgenic plants of A. sinensis in step three take root, the seedlings grow to a height of 3 cm for transplantation. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0022] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in Step 1, the promoter superpromoter is introduced into the vector p3300-Tnos to obtain the plasmid p3300-superpromoter-Tnos, and then the p3300-superpromoter-Tnos is extracted by alkaline lysis Plasmid, then use XbaI and SacI to carry out double enzyme digestion, the XbaI and SacI double enzyme digestion reaction system is 50 μL, consisting of 20 μL plasmid, 0.5 μL restriction endonuclease XbaI, 0.5 μL restriction endonuclease SacI, 5 μL 10× common enzyme digestion buffer and the balance of ddH 2 O composition; double enzyme digestion reaction: place in a water bath at 30°C for 2 hours. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a plant water treatment method of municipal wastewater at high altitude, relating to a water treatment method of municipal wastewater. According to the plant water treatment method, municipal wastewater is treated by adopting exotic invasive species, and oxygen combining capacity and oxygen requirement of herb of alligator alternanthera are enhanced by using a transgenic technology so that a municipal wastewater body with high pollution degree can be treated, the cold tolerance of the herb of alligator alternanthera can be improved, and thus the herb of alligator alternanthera can be applied to a high-altitude area so that the water treatment time in winter is prolonged, and the water treatment effect is improved. The plant water treatment method comprises the steps: 1, constructing a plant expression vector; 2, constructing a transgenetic seedling of the herb of alligator alternanthera; and 3, cultivating and breeding the transgenetic seedling of the herb of alligator alternanthera, and transplanting the transgenetic seedling of the herb of alligator alternanthera in a high-altitude polluted water body for performing wastewater treatment and remediation. The plant water treatment method is applied to the field of water environmental engineering.

Description

technical field [0001] The invention relates to a water treatment method for domestic sewage. Background technique [0002] Due to the rapid development of cities and the continuous increase of population, there are more and more domestic sewage in our country. According to the report of the State Environmental Protection Administration, the discharge of urban domestic sewage is increasing at an average annual rate of 5%, exceeding the discharge of industrial sewage. Domestic sewage not only affects water quality, but also poses a great threat to our living environment. [0003] Lotus seed grass (Latin scientific name: Alternantheraphiloxeroides (Mart.) Griseb.), aliases: revolutionary grass, water peanuts, lotus seed grass, etc., is a plant of the family Amaranthaceae. Perennial herb; stem base prostrate, upper part ascending, tubular, indistinctly 4-ribbed, 55-120 cm long, branched, young stems and leaf axils with white or rusty pilose, stems glabrous when old, only long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C12N15/82
CPCY02A90/40
Inventor 肖宇孙力高宇田媛
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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