Avian Encephalomyelitis virus attenuated vaccine strain

A technology of avian encephalomyelitis and virus, applied in the field of attenuated vaccine strains of avian encephalomyelitis virus, can solve the problem of low clinical incidence and achieve the effects of genetic stability and good commercial development prospects

Inactive Publication Date: 2014-04-09
YEBIO BIOENG OF QINGDAO
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Problems solved by technology

Almost all flocks will eventually become infected with the virus, but the clinical incidence is low

Method used

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  • Avian Encephalomyelitis virus attenuated vaccine strain
  • Avian Encephalomyelitis virus attenuated vaccine strain
  • Avian Encephalomyelitis virus attenuated vaccine strain

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0010] The present invention will be further described below in conjunction with specific embodiments.

[0011] 1. Breeding of avian encephalomyelitis virus strains

[0012] Take the brain, pancreas and duodenum of chicks with avian encephalomyelitis, grind them under sterile conditions, dilute them with sterilized normal saline at a ratio of 1:5, repeat freezing and thawing three times, centrifuge at 4000rpm for 30 minutes, and take the supernatant After filtering through the liquid filter, 10 1-day-old SPF chicks were inoculated in the brain, 0.1ml per chick. After symptoms such as ataxia and head and neck tremors appeared, blood was collected to kill them. Grind under sterile conditions, dilute 1:5 with sterilized saline, freeze-thaw 3 times, centrifuge at 4000rpm for 30 minutes, take the supernatant as the first-generation isolated virus (F1) and save it for later use. The F1 generation was inoculated into the brain of 1-day-old SPF chicks by the same method as above, and...

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Abstract

The invention provides an Avian Encephalomyelitis virus YBF02 strain. The strain, with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 8505, is preserved in China General Microbiological Culture Collection Center on November 8, 2013 at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The Avian Encephalomyelitis virus attenuated vaccine strain screened according the invention is subjected to continuous passage in a chicken body by horizontal transmitted infection, and no virulence return phenomenon occurs, therefore, the strain is stable in hereditary, and accords with the standard of no virulence return of Avian Encephalomyelitis virus attenuated vaccine strains; prepared vaccines can provide effective immune protection, and have a good commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of vaccine antigen breeding, and in particular relates to an attenuated vaccine strain of avian encephalomyelitis virus. Background technique [0002] Avian encephalomyelitis (AE) is a viral infectious disease caused by avian encephalomyelitis virus (AEV), which mainly harms chicks under 4 weeks of age, and infects the central nervous system and causes non-suppurative encephalitis as the main pathological feature. Manifested by neurologic symptoms such as ataxia, head and neck tremors, and numbness of the hind digits, especially tremors in the head and neck. Hence, it used to be called "epidemic tremor". After chickens under 4 weeks of age are infected with AEV, the incidence rate is generally 20% to 60%, and the mortality rate is 25% on average, which can also exceed 50%. Adult chicken infection does not show neurological symptoms, but it can cause a transient decline in egg production. The decline range ...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/125A61P31/14C12R1/93
Inventor 王红范根成杜元钊
Owner YEBIO BIOENG OF QINGDAO
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