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Naphthalene detecting biological cell sensor, as well as preparing method and use method thereof

A biological cell and sensor technology, applied in the field of naphthalene detection biological cell sensor and its preparation, can solve the problems of long detection time, complex components, low stability, etc.

Inactive Publication Date: 2014-04-02
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

The existing methods have complex components, low stability and long detection time

Method used

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  • Naphthalene detecting biological cell sensor, as well as preparing method and use method thereof
  • Naphthalene detecting biological cell sensor, as well as preparing method and use method thereof
  • Naphthalene detecting biological cell sensor, as well as preparing method and use method thereof

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Embodiment Construction

[0078] Such as figure 2 Shown, technical route of the present invention is as follows:

[0079] (1) Cultivation of light-sensing cell Acinetobacter ADPWH_lux (AcinetobacterADPWH_lux) for detection of salicylic acid

[0080] The previous work was to cultivate the obtained salicylic acid detection light-sensing cells ADPWH_lux.

[0081] (2) Acquisition of macromolecular fragment naphthalene degradation gene and vector plasmid

[0082] Firstly, the degradation mechanism of PAHs pollutants by degrading bacteria was analyzed. The desired degradative function bacteria or degradative plasmids with related functions can be obtained through existing reports. The choice of vector plasmid is to select the strain with strong promoter plasmid vector. Bacteria comprising the plasmid pDTG1 with the naphthalene degrading gene NahAD required in the examples - Pseudomonas NCIB9816, bacteria comprising the plasmid vector pWH1274 - Acinetobacter calcoaceticus and salicylic acid detection l...

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Abstract

The invention discloses a naphthalene detecting biological cell sensor, as well as a preparing method and a use method thereof. A gene recombination cell is used as the naphthalene detecting biological cell sensor, a salicylic acid detecting light sensing cell is used as a host cell of the gene recombination cell, and a plasmid of the gene recombination cell is recombined by a naphthalene degrading gene and a vector plasmid. The naphthalene detecting biological cell sensor can immediately emit light at the presence of naphthalene and has the advantages of great selectivity, high flexibility, high analysis speed, simplicity in operation, low instrument price and capability of carrying out continuous and stable online detection in a complicated system. The invention further provides a preparing method for the cell sensor. Adopted construction conditions are mild, the method is controllable, simple and feasible, the in-vitro recombination of a macro-molecular degrading fragment is finished by a Gibson cloning technology, and an important means is provided for the detection of a specific pollution substrate.

Description

technical field [0001] The present invention relates to a biological cell sensor and its preparation method and use. Specifically, the present invention relates to a naphthalene detection biological cell sensor and its preparation method and use method. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs-Polycyclic Aromatic Hydrocarbons), polycyclic aromatic hydrocarbons refer to a class of condensed ring compounds containing 2 or more benzene rings arranged in linear, angular or clustered shapes, with high melting point and boiling point, and hydrophobic There are more than 200 known polycyclic aromatic hydrocarbons. PAHs have the "three causes" effects of carcinogenicity, teratogenicity, and abruption. Because of their fat solubility, they are easily absorbed by the intestines and stomachs of animals, and can be enriched through the cascade amplification of the food chain and quickly dispersed to different places. function in the organization. Due to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/63G01N21/76C12R1/01
Inventor 孙寓姣赵晓辉丁爱中陈程张惠淳
Owner BEIJING NORMAL UNIVERSITY
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