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A kind of capsulated rhodobacter and its application

A technology of capsulated rhodobacter and bacteria agent, which is applied in the field of capsulated rhodobacter, can solve problems affecting consumers, high toxicity of sea cucumber, and affecting the growth of sea cucumber bait, so as to inhibit the growth of large algae, improve the micro-ecological environment, The effect of increasing income

Inactive Publication Date: 2016-02-03
大连市水产技术推广总站 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some farmers use chemical drugs in desperation. Although algae can be temporarily removed, this not only affects the growth of sea cucumber bait in the pond, but also has high toxicity to sea cucumbers. At the same time, the enriched chemical drugs will also affect the final consumer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, inhibit and remove the preparation of culture pond macroalgae bacterial agent

[0046]Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02gMgSO 4 ·7H 2 O, 0.02gK 2 HPO 4 , 0.1gNH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0047] Step 2: Sterilize the strain liquid culture medium at 120° C. for 15-20 minutes.

[0048] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of capsular rhodobacter as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for supplying the fermen...

Embodiment 2

[0052] Embodiment 2, inhibit and remove the preparation of culture pond macroalgae bacterial agent

[0053] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02gMgSO 4 ·7H 2 O, 0.02gK 2 HPO 4 , 0.1gNH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0054] Step 2: Sterilize the strain liquid culture medium at 120° C. for 15-20 minutes.

[0055] Step 3: Adopt a 200L fermenter, and carry out the following modification on the fermenter: (1) install an electric heating constant temperature control rod at the lower part of the fermenter; (2) set two external openings at the bottom of the fermenter, one as a vent for supplying the fermenter , and the other serves as an opening for adding culture medium, nutrients and strains.

[0056] Add 160L of liquid culture medium to the fermenter, and then inoculate Rhodobacter c...

Embodiment 3

[0059] Embodiment 3, inhibit and remove the preparation of culture pond macroalgae bacterial agent

[0060] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02gMgSO 4 ·7H 2 O, 0.02gK 2 HPO 4 , 0.1gNH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0061] Step 2: Sterilize the strain liquid culture medium at 120° C. for 15-20 minutes.

[0062] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of capsular rhodobacter as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for supplying the ferme...

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Abstract

The invention discloses rhodobacter capsulatus and an application thereof. The invention provides rhodobacter capsulatus BN-W-6 which has a collection number of CGMCC No.8311 and belongs to photosynthetic bacteria. The rhodobacter capsulatus is fermented by adding an energy source after enlargement cultivation to obtain fermentation products including chlorophyll a, carotenoid, ketone-carotenoid, hydrogenase, catalase and the like. Biological enzymes generated by the fermentation products of the rhodobacter capsulatus in a pond can ensure that macroscopic algae are gradually decomposed, the products including chlorophyll a, carotenoid and the like can absorb illumination wavebands necessary for growth of the macroscopic algae to make the macroscopic algae shrunk and agglomerated until death, and the dead macroscopic algae can not influence bottom matters, thereby achieving the aims of controlling rampant growth of algae and not influencing the normal growth of sea cucumbers. The fermentation broth of the rhodobacter capsulatus provided by the invention can be used for inhibiting and removing the macroscopic algae, so that the culture density is increased, the culture environment is improved, and the aim of improving the pond culture output of aquatic organisms is achieved.

Description

technical field [0001] The invention relates to a strain of capsulated rhodobacter and its application. Background technique [0002] As a pillar industry in the economy of the Yellow Sea and the Bohai Sea, sea cucumber farming plays a pivotal role in local economic development, promotion of fishermen's employment, and increase of fishermen's income. The flooding of macroalgae in ponds has always been the heart disease of sea cucumber farmers. Sea cucumber is one of the main aquatic products cultured in the Yellow Sea and Bohai Sea. The crazy growth of macroalgae has greatly hindered the efficient development of the aquaculture industry, and even threatened the existing industry survival. The reproduction of large algae has a great impact on the sea cucumber. The large algae occupy the living space of the sea cucumber, the activity space of the sea cucumber is reduced, and many of them are entangled by the large algae. The weeds are thrown away in the process of fishing, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 陈文博王伟李勃刘彤宋晓阳郝佳王骏
Owner 大连市水产技术推广总站
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