A kind of algae-dissolving/algae toxin-degrading double-effect engineering bacteria y7 and its construction method

Abstract

The invention provides a dual-effect engineering bacterium Y7 capable of lysing algae and degrading algal toxin and a construction method thereof, and belongs to the microbiological field of blue-green algae treatment. The dual-effect engineering bacterium Y7 capable of lysing algae and degrading microcystin-LR (MC-LR) is prepared based on a protoplast fusion technology in which the protoplast fusion rate can be up to 28.17% under optimal conditions. The dual-effect engineering bacterium Y7 provided by the invention is used for solving the problems of the outbreak of blue-green algae and the pollution of derived MC-LR, plays a guiding role in constructing a engineering bacterium integrating an MC-LR degradation function and algae-lysing property by virtue of an improved protoplast fusion technique and practical application and provides reference for solving the problem large-scale blue-green algae blooms fundamentally.

Description

technical field [0001] The invention belongs to the field of microbiology of cyanobacteria treatment, and in particular relates to a method for constructing alginolytic / algal toxin-degrading double-effect engineering bacteria. Background technique [0002] Excessive N, P and other substances enter the water environment, and the nutrients received by the water body exceed its own purification capacity, resulting in the excessive reproduction of cyanobacteria and other phytoplankton, and the accumulation of buoyant planktonic cyanobacteria on the surface of the water body, resulting in the phenomenon of "bloom". In addition, certain cyanobacteria, such as Microcystis aeruginosa, produce a large amount of algal toxins stored in the cells during the reproduction process, and are released with the death of the algal cells, and exist in the water in a soluble state, which seriously threatens the health of animals and humans. . There are more than 80 types of algae toxins that hav...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the pollution problem of cyanobacteria bloom and its derived algal toxins. In view of the fact that algae-dissolving bacteria dissolve the algae cells, the intracellular algae toxins will be released. The living habits of the strains are different, and the requirements for the environment are inconsistent. It is difficult to construct a complex flora that can not only dissolve algae but also solve the pollution problem of algal toxins derived from it, and make it adapt to the environment.

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