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Application of CpG-ODN 7909 sequence to preparation of drug for relieving tumor cell radiation tolerance

The technology of cpg-odn7909 and 1.cpg-odn7909 is applied in the field of the use of CpG-ODN 7909 sequence in the preparation of medicines for releasing radiation resistance of tumor cells, and can solve the problem that no CpG-ODN7909 sequence can release tumor radiation resistance and the like. problems, to achieve good therapeutic effects, broad application prospects, and increased sensitivity

Inactive Publication Date: 2014-03-26
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on how CpG-ODN 7909 sequence relieves tumor radiation tolerance

Method used

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  • Application of CpG-ODN 7909 sequence to preparation of drug for relieving tumor cell radiation tolerance
  • Application of CpG-ODN 7909 sequence to preparation of drug for relieving tumor cell radiation tolerance
  • Application of CpG-ODN 7909 sequence to preparation of drug for relieving tumor cell radiation tolerance

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Experimental program
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Effect test

Embodiment 1

[0021] 1 Experimental materials

[0022] Human lung adenocarcinoma A549 cells, CpG-ODN 7909 sequence synthesized by Shanghai Sangon, CCK-8 kit for detecting cell proliferation and cytotoxicity, cell cycle detection kit, western series consumables, primary antibodies TOLL-9, β -actin, secondary antibodies against TOLL-9 and β-actin, 1640 medium, etc.

[0023] 2 Experimental methods

[0024] 2.1 Culture A549 cells

[0025] A549 cells were cultured in 1640 medium containing 10% newborn bovine serum and 1% double antibody at 37°C, 5% CO 2 cultured in an incubator.

[0026] 2.2 Obtaining the radiation-resistant cell line R-549 cells

[0027] A high-energy linear accelerator was used to irradiate 6MV X-rays. The irradiation field was 7 cm×7 cm, the source-to-skin distance was 100 cm, and the absorbed dose rate was 200 Gy / min. The parental A549 cells in the exponential growth phase were irradiated with a dose of 637 Gy and continued to be cultured. When the cells reached the end...

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Abstract

The invention provides an application of a CpG-ODN 7909 sequence to the preparation of a drug, i.e., a radiosensitizer for relieving tumor cell radiation tolerance. The CpG-ODN 7909 sequence is 5'-TCGTCGTTTTGTCGTTTTGTCGTT-3' and takes non-methylated cytosine guanine dinucleotide as a core. The CpG-ODN 7909 sequence is proved to be capable of remarkably reducing the growth speed of a radiation-resistant cell system, delaying the cell period, enhancing the radiation sensibility and lowering the radiation tolerance with the assistance of radiation, so that the CpG-ODN 7909 sequence can be used as the radiosensitizer to treat tumor with the assistance of radiation, can be used for achieving a favorable treating effect and has a wide application prospect.

Description

technical field [0001] The present invention relates to the new use of CpG-ODN 7909 sequence, specifically, the use of CpG-ODN 7909 sequence in preparing medicine for releasing tumor cell radiation tolerance. Background technique [0002] In recent years, the incidence of lung cancer has shown an obvious upward trend, and the traditional treatment methods are not effective. Among them, radiotherapy plays a pivotal role in the treatment of lung cancer, but the probability of tumor radiotherapy failure is high. The main reason is that tumor cells are resistant to radiation. the existence of receiving. Therefore, finding a way to relieve the radiation tolerance of tumor cells will have far-reaching significance for the treatment of lung cancer. Exploring effective radiosensitizers has also attracted much attention in the field of tumor research for many years. [0003] Radiation sensitizers refer to drugs that do not kill cells when used alone, and can only increase the killin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61P35/00A61P11/00A61P43/00
Inventor 乔田奎燕丽陈伟袁素娟
Owner JINSHAN HOSPITAL FUDAN UNIV
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