Tissue culturing method of radix zanthoxyli

Abstract

The invention relates to a tissue culturing method of radix zanthoxyli and belongs to the technical field of crop planting. The tissue culturing method comprises six steps of material pretreatment, inoculation, culture, transplantation, cultivation management and seedling delivery out of a garden. The tissue culturing method comprises the specific steps of: on a fine day, selecting a good plant with strong growth and no disease and pest damage, washing with tap water, soaking the plant for 6-8 minutes in potassium permanganate solution, washing for 2-3 times with aseptic water, cutting the plant into small sections which are 0.5-0.8cm in length and have sprouts under the aseptic condition, inoculating the small sections on a culture medium under the aseptic condition, and culturing for 4-5 days under the condition with the temperature being 30-35 DEG C; after the culture is complete, carrying out seedling exercise for 3-5 days on seedlings under natural environment, transplanting the seedlings onto a matrix after adapting to the natural condition, and carrying out cultivation on the seedlings under the condition with the temperature being 25-35 DEG C and the humidity being 50-70%; after transplanting, watering for maintaining the soil to be wet, applying fertilizers once every week, and after 4-5 months, when the height of the seedlings is more than 20cm, the seedlings can be delivered out of the garden for planting. The success rate of the radix zanthoxyli planted by adopting the tissue culturing method is highest and is up to more than 95%; the culturing method is simple, is short in culture time and is beneficial to large-scale production.

Description

technical field [0001] The invention relates to a method for tissue culture of plants, in particular to a method for tissue culture of double-faced needles with multiple functions, and belongs to the technical field of crop cultivation. Background technique [0002] Zanthoxylum nitidum (Roxb.) DC. was first recorded in the "Shen Nong's Materia Medica" under the name of Manjiao, and was listed as a lower grade. Zhao Qiguang's "Materia Medica Seeking the Original" in the Qing Dynasty named it Jinniu. According to the textual research of Materia Medica, it is the dry root and stem of the woody vines of the family Rutaceae (Rumceae) and the genus Zanthoxylum. It has been included in the "Chinese Pharmacopoeia" since 1977. Liangmianzhen has a long history of medicinal use in my country, and is widely used and used in large quantities. It is one of the commonly used traditional Chinese medicinal materials in the southern region and is mainly distributed in Guangxi, Fujian, Hunan, ...

AI Technical Summary

Problems solved by technology

[0005] There are three methods for cultivating L. chinensis seedlings: seed propagation, cutting propagation and tissue culture. Document CN101099433B discloses a method for propagating the plant L. chinensis. The cutting bed is covered with sandy soil, and the lignified cuttings are rested and directly Cutting, transplanting after half a year to one year; document CN102986437A discloses a method of artificial cultivation of double-faced needles, using seedlings or nutrition cup seedlings or cuttings to raise seedlings, with high yield and stable product quality; and for the organization of double-faced needles There are few researches on cultivation methods. The present invention conducts in-depth research on the tissue culture method of L. chinensis, and provides a new method for artificial cultivation of L. chinensis, with high survival rate and good growth