Method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater

A Bacillus subtilis and amino acid technology, which is applied in the field of comprehensive utilization of wastewater resources, can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation cost, and achieves high nitrogen source utilization efficiency and high application promotion. Potential, the effect of strong competitive advantage

Inactive Publication Date: 2014-03-19
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported amino acid wastewater treatment methods mainly include electrodialysis, ultrafiltration, biochemical methods, etc., but the shortcomings of electrodialysis and ultrafiltration limit the promotion of this method due to high investment and operating costs and inconvenient maintenance; and biochemical methods Because of its low investment and operating costs, mature technology, no secondary pollution, and convenient operation and management, it has received worldwide attention in organic wastewater treatment.
The problem with this method at present is that it is more suitable for medium and low concentration sewage, but it is not suitable for treating high concentration amino acid wastewater
However, the whole treatment method has the disadvantages of high cost, time-consuming and labor-intensive concentration process, disconnection between resource recovery and wastewater treatment, and the fact that organic matter is easy to deteriorate after high-temperature evaporation and concentration.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Breeding of Bacillus subtilis strains for feed.

[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:

[0027] Table 1 Contents of related elements in amino acid wastewater

[0028] project name

unit

Test results

Method basis

Total Nitrogen (N)

％

8.79

GB / T8572-2010

Sodium (Na)

％

0.52

ICP method

Chlorine (Cl)

g / L

131.26

AgCl nephelometric method

Lead (Pb)

mg / kg

0.91

ICP method

Arsenic (As)

mg / kg

0.076

Atomic Fluorescence

Mercury (Hg)

mg / kg

0.014

Atomic Fluorescence

Chromium (Cr)

mg / kg

1.51

ICP method

Cadmium (Cd)

mg / kg

0.06

ICP method

[0029] Table 2 Composition and content of amino acids in amino acid wa...

Embodiment 2

[0037] Fermentation of Bacillus subtilis in liquid medium with glucose as carbon source and amino acid wastewater as nitrogen source.

[0038] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: glucose: 50g / L, KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 7.2.

[0039] 2. Inoculate the liquid fermentation medium prepared in step 1 by the domesticated Bacillus subtilis bacterial strain obtained from embodiment 1 by 2% inoculum, in the fermentor at 31 ° C, the rotating speed is 220rpm / min, and the aeration coefficient is Under the condition of 0.5, the liquid fermentation was cultivated for 2 days. After the cul...

Embodiment 3

[0041] Fermentation of Bacillus subtilis in liquid medium with enzymatic hydrolysis of corn starch as carbon source and amino acid wastewater as nitrogen source.

[0042] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: corn starch enzymatic hydrolysis solution (according to the dry weight of corn starch): 50g / L, KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 7.2.

[0043] 2. inoculate the liquid fermentation medium prepared in step 1 by the acclimated Bacillus subtilis bacterial strain obtained from embodiment 1 by 5% inoculum, in the fermentor at 31 ℃, the rotating speed is 220rpm / min, and the aeration coeffi...

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Abstract

The invention discloses a method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater, which comprises the following steps: 1. habituated culture of feed strain: carrying out continuous habituated culture to enhance the tolerance of the strain to high-salt-content amino acid wastewater; and 2. fermentation of strain: under appropriate fermenting conditions, carrying out liquid ventilation culture in a fermentation tank for some time to obtain the Bacillus subtilis for feeds. The microzyme is centrifugated, collected, dried in a 70 DEG C drying oven to constant weight, and weighed. The calculation indicates that every liter of high-salt-content amino acid wastewater can obtain 35g of Bacillus subtilis at the maximum. The method can effectively utilize amino acids, microelements and growth factors contained in the wastewater, greatly lowers the fermenting cost of the Bacillus subtilis for feeds, greatly reduces the sewage treatment load, achieves the comprehensive utilization effect, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of waste water resources, in particular to a method for fermenting high-salt amino acid waste water to produce Bacillus subtilis for feed. Background technique [0002] According to the World Health Organization, 70% of people in the world do not have access to safe and hygienic drinking water, and 25,000 people die every day from diseases related to sewage or poor sanitation; by 2050, it is estimated that there will be about 700,000 people in the world Millions of people face water shortages. 82% of the people in our country drink water from shallow wells and rivers, 75% of which are polluted by bacteria exceeding the hygienic standard, and the drinking water population polluted by organic matter is about 160 million. The sewage discharged by industry is the main source of water pollution. According to survey statistics, the national annual wastewater discharge is 65.92 billion tons. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 杨波胡征刘志刚
Owner HUBEI UNIV OF TECH
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