A kind of fermented milk and preparation method thereof
A fermented milk and starter technology, applied in dairy products, milk preparations, applications, etc., can solve problems such as loose texture, serious water separation, and unsatisfactory stability, and achieve good water retention, appropriate viscosity, and taste smooth rich effect
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Embodiment 1
[0031] 1) Take the Leuconostoc mesenteroides CGMCC NO.6432 stored at -80°C, activate it on a petri dish containing 5% sucrose M17 solid medium, and put it in sterile skim milk containing 5wt% sucrose Expand cultivation step by step to obtain fermented seed liquid (cultivation temperature 28°C), and the cultivation time is 32 hours;
[0032] 2) Heat and mix raw materials including the following weight percentages: 90% fresh milk, 10% white sugar, heating temperature is 40°C, homogenize while hot (20MPa), sterilization treatment, sterilization temperature is 95°C, sterilization time is 5 minutes ;
[0033] 3) Inoculate the fermented seed solution obtained in step 1) into the aseptic fresh milk obtained in step 2) above, so that the number of Leuconostoc enterocolitica colonies at the beginning of fermentation reaches 1×10 6 cfu / mL, fermented at 25°C for 48 hours to obtain Leuconostoc enterococci fermented milk.
[0034] 4) Produce conventional fermented milk with ordinary tech...
Embodiment 2
[0037] 1) Activate Leuconostoc mesenteroides CGMCC NO.6432 according to the same method as in Example 1 to obtain a fermented seed liquid;
[0038] 2) Heat and mix the raw materials including the following weight percentages: fresh milk 82%, white sugar 18%, heating temperature is 45°C; homogenize while hot (10MPa), sterilization treatment, sterilization temperature is 90°C, sterilization time is 10 minutes ;
[0039] 3) Inoculate the fermented seed solution obtained in step 1) into the aseptic fresh milk obtained in step 2) above, so that the number of Leuconostoc enterocolitica colonies at the beginning of fermentation reaches 1×10 8 cfu / mL, fermented at 33°C for 15 hours to obtain Leuconostoc enterococci fermented milk.
[0040] 4) Produce conventional fermented milk with ordinary technology: fresh milk (heated to 50°C) + white sugar → high-speed stirring for 20 minutes → heating to 60°C ~ 65°C → homogeneous [primary pressure (16.5±0.5) MPa, secondary pressure Step pressu...
Embodiment 3
[0043] 1) Activate Leuconostoc mesenteroides CGMCC NO.6432 according to the same method as in Example 1 to obtain a fermented seed liquid;
[0044]2) Heat and mix the raw materials including the following weight percentages: fresh milk 95%, white sugar 5%, heating temperature is 50°C; homogenize while hot (15MPa), sterilization treatment, sterilization temperature is 88°C, sterilization time is 8 minutes ;
[0045] 3) Inoculate the fermented seed solution obtained in step 1) into the above-mentioned aseptic fresh milk, so that the number of colonies of Leuconostoc enterocolitica reaches 1×10 at the beginning of fermentation. 8 cfu / mL, fermented at 30°C for 20 hours to obtain Leuconostoc enterococci fermented milk.
[0046] 4) Produce conventional fermented milk with ordinary technology: fresh milk (heated to 50°C) + white sugar → high-speed stirring for 20 minutes → heating to 60°C ~ 65°C → homogeneous [primary pressure (16.5±0.5) MPa, secondary pressure Step pressure (3.5±0...
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