Preparation for fat-reducing peptide
A production method and fish meat technology, which are applied in food preparation, protein composition of fish, food science and other directions, can solve the problems of unstable weight loss effect, side effects that cannot be ignored, and single type of drugs, so as to improve the antioxidant level of the liver and improve the Liver damage, easily absorbed effect
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[0013] (1) Pretreatment of fish meat: the crucian carp is descaled, skinned, head and tail removed, impurities removed, cut into fish pieces, after washing, the fish meat is chopped with a grinder, preserved, and set aside.
[0014] (2) Extraction of water-soluble protein from fish meat: mix the crushed fish meat with water in a ratio of 1:9, stir and extract at 40~43℃ for 2.5~3h, centrifuge at 4000r / min for 10~15min, take the supernatant to obtain the water solubility Protein extract.
[0015] (3) Preparation of weight loss peptide: Mix the alkaline protease with the water-soluble protein extract of fish meat at a ratio of 100 U / mL. After enzymolysis at 38~42℃ for 8~11h, boil the enzyme at 100℃ for 10~15min, 4000r / After centrifugation for 10 minutes, the upper enzymatic hydrolysate is treated by ultrafiltration (MWCO=5KDa), and then the fractions with molecular weight less than 5KDa are concentrated by vacuum rotary evaporation, and dried to obtain slimming peptide products.
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