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Immune chromatography result identification method based on envelope area and twice compensation

A technology of envelope area and immunochromatography, applied in the field of immunochromatography result recognition based on envelope area and double compensation, can solve problems such as few quantitative analysis methods, and achieve the effect of improving accuracy

Inactive Publication Date: 2014-03-05
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
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Problems solved by technology

However, for the fluorescence luminescence detected by immunochromatography, there are still few quantitative analysis methods

Method used

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  • Immune chromatography result identification method based on envelope area and twice compensation
  • Immune chromatography result identification method based on envelope area and twice compensation
  • Immune chromatography result identification method based on envelope area and twice compensation

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Embodiment Construction

[0037] Hereinafter, the present invention will be described in detail with reference to the accompanying drawings and the embodiments.

[0038] Reference figure 1 As shown, an immunochromatographic result identification method based on envelope area and twice compensation includes the following steps:

[0039] Step 1) Acquire the original waveform

[0040] Collect the original waveform of immunochromatographic fluorescence waveform;

[0041] Step 2) Smoothing and filtering

[0042] Using the mean filtering method, noise filtering is performed on the immunochromatographic fluorescence waveform to obtain a clearer fluorescence waveform. In this embodiment, 1850 point sampling is performed on the immunochromatographic fluorescence waveform: that is, N=1850, to obtain image 3 The coordinate curve shown is for the amplitude sequence of the fluorescence waveform , Using three-point average filtering for smoothing filtering, the formula is:

[0043] ;

[0044] Step 3) Background threshold p...

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Abstract

The invention discloses an immune chromatography result identification method based on envelope area and twice compensation. The method is as below: first conducting smoothing filtering on collected immune chromatography fluorescence waveform to clear the fluorescence waveform; then conducting background threshold treatment on the waveform after filtering to remove the background signals in the waveform; and calculating a ratio of a detection line waveform envelope area and a quality control line waveform envelope area, conducting waveform width compensation and temperature compensation to finally obtain detection result of immune chromatography fluorescence. The invention uses a method of calculating envelope area ratio to realize automatic accurate detection of the immune chromatography fluorescence results; the twice compensation method combining the wave width compensation and temperature compensation to further improves the accuracy of immune chromatography fluorescence detection.

Description

Technical field [0001] The invention belongs to the field of immunochromatographic detection, and specifically relates to an immunochromatographic result identification method based on envelope area and twice compensation. Background technique [0002] Immunoassay is a method that uses antigen-antibody reaction to detect trace substances in specimens. Based on the specificity and sensitivity of the antigen-antibody reaction, immune detection methods have been widely used in many fields such as medical detection and biological detection. Any substance can be detected by immunoassay as long as the corresponding specific antibody can be obtained. [0003] The immunochromatographic detection method is the product of a combination of immunoassay methods and chromatography analysis techniques. In this method, specific antibodies are sprayed on a hydrophilic chromatography test strip, and the sample to be tested is dropped on one end of the chromatography test strip during detection. W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/558
Inventor 罗刚银
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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