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Louisiana iris hybrid seed germination method

A hybrid seed and iris technology, applied in seed immunization, seed and rhizome treatment, application, etc., can solve the problems of irregular emergence, hindrance to application, low reproduction coefficient, etc., shorten the emergence time, shorten the germination time, and increase the germination rate Effect

Inactive Publication Date: 2014-03-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the natural reproduction coefficient of Iris Louisiana is low, and the germination rate of hybrid seeds is low under natural conditions, and the emergence of seedlings is irregular.
The reason is that the hybrid seeds of most species of the genus Iris have dormancy characteristics, which hinders their application in horticultural cultivation and brings certain difficulties to the hybrid breeding work.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Formula of solid medium: MS medium 2.2g / L, sucrose 60g / L, agar 6g / L, BA0.5mg / L.

[0015] (2) Select the dehiscated Louisiana iris hybrid capsules, wash the seeds in the capsules with tap water for 2 hours, soak them in sterile water for 24 hours; then sterilize them with 95% alcohol for 60 seconds, and then put them in a solution of 2% sodium hypochlorite Disinfect for 10 minutes, and finally rinse with distilled water.

[0016] (3) Carefully cut the plump hybrid seeds along the ventral suture on the ultra-clean workbench, peel off the complete embryos, transfer them to the above solid medium, and inoculate 9 embryos per dish. Then it was placed in a constant temperature aseptic culture room at 25°C for dark culture. After 24 hours, one end of the radicle began to expand, and after 48 hours, the embryos turned yellow-green. After 4 days, the embryos were bent geotropically, and the germination rate of the embryos reached 87.6%. The vigorously growing buds were tran...

Embodiment 2

[0018] (1) Formula of solid medium: MS medium 2.2g / L, sucrose 53g / L, agar 4g / L, BA0.8mg / L.

[0019] (2) Harvest mature Louisiana iris hybrid capsules that have not been separated and cracked, gently scrub the harvested hybrid capsules with a soft brush, and rinse them in tap water containing 0.2% Tween and detergent for 2 hours. Then disinfect it with 85% alcohol for 60 seconds, put it into a solution of 2% sodium hypochlorite for 10 minutes, and finally rinse it with distilled water.

[0020] (3) Cut the sterilized hybrid capsules on the ultra-clean workbench, select the plump hybrid seeds and carefully cut them along the abdominal suture, peel off the complete embryos, transfer them to the above solid medium, and inoculate 9 Granules. Then it was placed in a constant temperature aseptic culture room at 25°C for dark culture. After 24 hours, one end of the radicle began to expand, and after 48 hours, the embryos turned yellow-green. After 4 days, the embryos were bent geotro...

Embodiment 3

[0022] (1) Formula of solid medium: MS medium 2.2g / L, sucrose 50g / L, agar 5g / L, BA1mg / L.

[0023] (2) On the ultra-clean workbench, carefully dissect the Louisiana iris hybrid seeds that have been sown for 60 days but have not germinated along the ventral suture, peel off the complete embryos, transfer them to the above solid medium, and inoculate 9 per dish. Granules. Then it was placed in a constant temperature aseptic culture room at 25°C for dark culture. After 24 hours, one end of the radicle began to expand, and after 48 hours, the embryos turned yellow-green. After 4 days, the embryos were bent geotropically, and the germination rate of the embryos reached 82.5%. The vigorously growing buds were transferred to cylindrical flasks with the same medium for light cultivation. The cultivation temperature was 25°C, the light intensity was 1500Lx, and the daily light time was 12h / d; after 20 days, the seedling height was 8cm, and the seedlings were hardened and transplanted. ...

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PUM

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Abstract

The invention relates to a seed germination technology, and provides a method for Louisiana iris hybrid seed germination. The method comprises the following steps: immersing the matured Louisiana iris hybrid seeds in sterile water for 24 hours, sucking water for satiation and disinfecting, stripping a complete embryo for inoculating in a solid medium for in-vitro culture in dark, inoculating to the solid medium of a same component after the embryo is germinated, and then illuminating for culturing, and acquiring the hybrid seed. The method of the invention employs an embryo culture technology to perform in-vitro culture on the hybrid seeds in period of dormancy, the hybrid seeds can break the dormancy, and the embryo enables rapid expansion and elongation in the medium. Compared with a routine sowing method, the germination rate in the invention increases by 60%, the germination time shortens by 12 days, seedling emergence time shortens by 20 days, the operation is simple, and the workload is not increased.

Description

technical field [0001] The invention relates to seed germination technology, in particular to a method for Louisiana iris hybrid seed germination. Background technique [0002] Louisiana Iris is a perennial evergreen perennial aquatic flower of the family Iridaceae. It has large flowers, rich and gorgeous colors, various flower types, and strong adaptability. Its leaves remain evergreen in winter in East China and can be used as an excellent material for hybrid breeding. However, the natural reproduction coefficient of Iris Louisiana is low, and the germination rate of hybrid seeds is low under natural conditions, and the emergence of seedlings is irregular. The reason is that the hybrid seeds of most species of the genus Iris have dormancy characteristics, which hinders their application in horticultural cultivation and brings certain difficulties to the hybrid breeding work. [0003] The main factors causing dormancy in Iris hybrid seeds are the effects of the seed coat a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
Inventor 王冠群夏宜平李丹青张佳平
Owner ZHEJIANG UNIV
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