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Verticillium dahliae culture medium and preparation method thereof, and method for culturing verticillium dahliae

A technology of Verticillium dahliae and culture medium, applied in the field of agricultural biology, can solve the problem of low spore production and achieve the effect of increasing spore production

Active Publication Date: 2014-02-26
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the course of research on Verticillium dahliae, it is often necessary to use a large amount of Verticillium dahliae spores, however, the media currently used for cultivating Verticillium dahliae (such as Chapik medium) are not suitable for culturing Verticillium dahliae Sporulation is still low when mycobacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0015] The present invention also provides a preparation method of Verticillium dahliae culture medium, wherein the method includes: (1) quick-freezing cotton tissue with liquid nitrogen, crushing and drying to obtain cotton tissue powder; (2) The cotton tissue powder and NaNO 3 , KCl, K 2 HPO 4 , FeSO 4 And MgSO 4 Mix and prepare to contain 1-3g / L NaNO 3 , 0.3-0.7g / L KCl, 0.5-2g / L K 2 HPO 4 , 0.005-0.015g / L FeSO 4 , 0.3-0.7g / L MgSO 4 And 5-15g / L cotton tissue powder of Verticillium dahliae culture medium.

[0016] Among them, in order to further increase the spore production of Verticillium dahliae, preferably, the cotton tissue powder is combined with NaNO 3 , KCl, K 2 HPO 4 , FeSO 4 And MgSO 4 Mix and configure to contain 1.5-2.5g / L NaNO 3 , 0.4-0.6g / L KCl, 0.8-1.2g / L K 2 HPO 4 , 0.008-0.012g / L FeSO 4 , 0.4-0.6g / L MgSO 4 And 8-12g / L cotton tissue powder.

[0017] Wherein, the cotton tissue powder can be derived from various components of a cotton plant, including but not limited ...

preparation Embodiment 1

[0024] Cottonseed (Hefeng 202, purchased from Shijiazhuang Wanfeng Seed Industry Co., Ltd.) was planted in commercial vermiculite and nutrient soil (purchased from Beijing Fanxing Flower Co., Ltd.), cultivated in a greenhouse (photoperiod 14h, temperature 25°C, relative humidity 85% ) 4-6 weeks old cotton seedlings are obtained.

[0025] The 4-6 weeks old cotton seedlings obtained above are cleaned, the roots and stems of the cotton plants are taken, cut into pieces, quick-frozen in liquid nitrogen and broken into powder, and then dried to obtain cotton tissue powder for use.

Embodiment 1

[0027] 2g of NaNO 3 , 0.5g of KCl, 1g of K 2 HPO 4 , 0.01g FeSO 4 , 0.5g of MgSO 4 Mix with 10g of the cotton tissue powder of Preparation Example 1, and add water to 1L, and after sterilization, the Verticillium dahliae culture medium of this example is obtained.

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Abstract

The invention discloses a verticillium dahliae culture medium, which contains 1-3g / L of NaNO3, 0.3-0.7g / L of KCl, 0.5-2g / L of K2HPO4, 0.005-0.015g / L of FeSO4, 0.3-0.7g / L of MgSO4 and 5-15g / L of cotton tissue powder. The invention also provides a preparation method of the verticillium dahliae culture medium and a method for culturing verticillium dahliae. According to the invention, the spore yield of the verticillium dahliae can be increased by about 5 times.

Description

Technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a Verticillium dahlia culture medium, a preparation method of a Verticillium dahlia culture medium and a method for cultivating Verticillium dahlia. Background technique [0002] Verticillium dahlia is a fungus belonging to the genus Verticillium dahlia. The host range of Verticillium dahliae is extremely wide and can damage 660 species of plants in 38 families, including 184 species of crops and 153 species of weeds. In China, the host plants of Verticillium dahlia involve at least 80 species in 20 families, such as sunflower, eggplant, pepper, tomato, tobacco, potato, melon, watermelon, cucumber, peanut, kidney bean, mung bean, soybean, cotton, sesame, beet Waiting for field crops. Among them, Verticillium dahliae can cause Verticillium wilt in cotton, so it is of great significance to study the biological characteristics of Verticillium dahliae. [0003] In the research p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 戴小枫肖红利李蕾陈捷胤祁伟彦孔志强
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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