Method for producing beta-carotene by solid-state fermentation of sitophila vein bacterium

Abstract

The present invention discloses solid N. sitophila fermenting process of producing beta-carotene. The process adopts N. sitophila strain No. 17 as the producing bacteria, culture medium compounded with soybean residue and wheat bran, and optional fermenting assistant(s) or fermenting promoter(s) in solid state fermentation with or without lighting for aerobic culturing. The fermented product is treated through breaking spore wall with hot hydrochloric acid, extracting with acetone extractant, rotary evaporation to eliminate organic solvent and chromatographic separation and purification in alumina or magnesia column to compound beta-carotene product. The present invention has the advantages of optimized bacterial strain, simple production process, no pollution, etc.

Description

A method for producing β-carotene by solid-state fermentation of Neurospora california technical field The invention relates to a method for producing beta-carotene by solid-state fermentation of Neurospora gourami. Background technique β-carotene is a Class A excellent nutritional food additive recognized by the Food and Agriculture Organization of the United Nations and the World Health Organization Joint Expert Committee on Food Additives. The world's annual demand is more than 1,000 tons, but the current global production is only about 600 tons, and the market has great potential for development . Considering the economy, if the synthetic β-carotene is calculated at 400 US dollars / kg, it is estimated that the world's annual sales will reach 200-250 million US dollars, and the development prospect of economic benefits is also very considerable. At present, there are three main production methods of natural β-carotene. The first one is to extract directly from natural ...

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Problems solved by technology

Several companies such as Natritite in the United States and Betatene in Australia produce natural β-carotene through breeding Dunaliella salina, with an annual production capacity of more than 30 tons. However, the cultivation of salina is strictly limited by external environmental conditions, making it difficult to expand the scale. , far from meeting the market demand