A kind of microecological solid preparation for treating alcoholic liver injury and preparation method thereof
A technology for alcoholic liver injury and solid preparation, which is applied in the field of microecological solid preparation and its preparation, can solve the problems of general effect, increased production cost of compound bacteria, short survival time of probiotics, etc., and achieves obvious effect and suitable scale. Production, high yield effect
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Embodiment 1
[0023] Example 1. Production process of Bifidobacterium longum NQ-1501 strain powder
[0024] The Bifidobacterium longum NQ-1501 strain of Shuangqi Company was selected as the only strain of the preparation.
[0025] The best fermentation medium for Bifidobacterium longum strain NQ-1501: 1.2% lactose, 0.8% beef extract, 0.4% triamine citrate, 0.4% potassium acetate, 0.04% magnesium sulfate, 0.05% L-cysteine salt Salt, 0.08% Tween-80.
[0026] Bifidobacterium longum NQ-1501 is cultured anaerobic in its fermentation medium, the fermentation temperature is 40℃, and the optimal fermentation time is 8h. Centrifuge the obtained fermentation broth at 15,000 g for 30 minutes, discard the supernatant to obtain a wet bacterial sludge, add a protective agent, stir evenly, and pour into a freeze dryer to freeze-dry to obtain a white powdery bacterial powder. The formula of the protective agent is: add 20ml of 60% skimmed milk powder and 5ml of 5% starch in a 300ml system.
Embodiment 2
[0027] Example 2. Production of microecological solid preparation for treating alcoholic liver injury
[0028] Mix Bifidobacterium longum NQ-1501 powder and auxiliary material starch in a ratio of 1:9 to make tablets, capsules and powders.
[0029] a. Mix Bifidobacterium longum NQ-1501 powder and auxiliary material starch in a ratio of 1:9, and use a tablet press to form flat tablets on both sides. The average tablet weight is 0.4g, and the tablet weighs 1g. Live bacteria The number is about 20 billion, and the dosage for adults is 2 tablets a day, and a course of treatment is 30 days.
[0030] b. Mix Bifidobacterium longum NQ-1501 powder and auxiliary material starch in a ratio of 1:9 and put them in a capsule in a cylindrical shell made of gelatin. The volume of the capsule is 0.48ml. , The number of viable bacteria per capsule is about 20 billion, and the dosage for adults is 2 capsules per day, and 30 days is a course of treatment.
[0031] c. Mix the Bifidobacterium longum NQ-15...
Embodiment 3
[0032] Example 3. Hepatoprotective properties of microecological solid preparation for treating alcoholic liver injury
[0033] 1. Experimental materials:
[0034] 1. Experimental preparation:
[0035] The microecological solid preparation containing Bifidobacterium longum NQ-1501 for the treatment of alcoholic liver injury was provided by Inner Mongolia Shuangqi Pharmaceutical Co., Ltd.
[0036] 2. Experimental animals:
[0037] Adult Wistar male mice, weighing 18±22 grams, were provided by the Animal Experiment Center of Inner Mongolia University.
[0038] 3. Experimental kit:
[0039] Triglyceride (TG enzymatic method) test kit, serum very low density lipoprotein (VLDL) ELISA kit.
[0040] 2. Experimental method:
[0041] The mice were randomly divided into 5 groups, namely the blank control group, the model group, and the test sample group. The test sample group was divided into high (90mg / kg.BW), medium (45mg / kg.BW), and low. (22.5mg / kg.BW) dose group, each group has 10 rats. The tes...
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