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Active transposon of fish Tc1-like and application of active transposon

A technology for ptgf2-mlyz2-rfp and gene transfer, applied in the field of genetic engineering

Inactive Publication Date: 2015-05-27
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the face of problems such as the stability and safety of transgenes, only by further in-depth research on the transposon system and transposition mechanism and the influence of host factors on its transformation efficiency in the future can the development of transgenic technology be better promoted

Method used

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  • Active transposon of fish Tc1-like and application of active transposon
  • Active transposon of fish Tc1-like and application of active transposon
  • Active transposon of fish Tc1-like and application of active transposon

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Experimental program
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Effect test

Embodiment 1

[0044] 1. Fish Tc1 -Like transposon acquisition and analysis

[0045] 1 Collection and processing of experimental materials

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Abstract

The invention relates to a fish Tc1-like active transposon and its application. Specifically provided are transposons with complete left and right terminal inverted repeat sequences, transposons capable of encoding transposases and transposases encoded therein, gene vectors and gene transfer systems containing the above transposons and transposases. Through the verification of transposition activity in zebrafish, it is confirmed that the above-mentioned transposon has transposition activity, and the gene transfer system can perform high-efficiency transposition in zebrafish, so the present invention is to further verify the transposition efficiency of transposon And the use of the transposon in vertebrate related research and application has laid a foundation.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a fish Tc1 -like active transposons and their uses. Background technique [0002] Transposons are a class of DNA sequences that can jump and move within and between genomes without relying on homologous recombination, also known as jumping genes or mobile elements, which are widely distributed in nature. According to their transposition mechanism, they are divided into two categories: retroposons and DNA transposons. Tc1 -like transposons are the most diverse and widely distributed type of DNA transposon family. since Tc1 After transposons were discovered in nematodes, and Tc1 Transposons with similar structures collectively Tc1 -like transposons, whose family structure is characterized by a gene encoding a transposase with terminal inverted repeats at both ends. Tc1 -like transposons are divided into two types because of different transposition activities: auto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/52C12N9/00C12N15/63
Inventor 邹曙明郭秀明蒋霞云
Owner SHANGHAI OCEAN UNIV
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