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Separation purification method of refined heparin sodium

A technology for separation and purification of heparin sodium, which is applied in the field of separation and purification of high-quality heparin sodium, which can solve the problems of difficult removal of impurities, low product quality, and low purity, so as to ensure quality and activity, reduce production costs, and improve the purity of high-quality products Good results

Inactive Publication Date: 2014-02-19
ANHUI IND & TRADE VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the crude heparin sodium produced in China often contains more impurities, the purity is low, and the product quality is not high. However, it is difficult to remove these impurities in the crude product heparin sodium, which increases the impact on subsequent further purification into medicinal heparin sodium. Due to the difficulty, the obtained medicinal heparin sodium products are often of poor quality and difficult to meet the requirements

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] A method for separating and purifying high-quality heparin sodium, comprising the steps of:

[0025] (1) Salt solution:

[0026] Take the aqueous solution of crude heparin sodium, add NaCl to make the NaCl content 4wt%, adjust the pH value to 9-10, salt it at 40°C for 2 hours, cool it down to 10°C, let stand, centrifuge, and collect the supernatant;

[0027] (2) Adsorption and elution:

[0028] Take the supernatant obtained in step (1) and the pretreated D-254 anion resin and stir for 2 hours at a stirring speed of 60r / min, filter and discard the filtrate; wash the anion resin with 3wt% NaCl aqueous solution; then use 12%wtNaCl aqueous solution Soak the anion resin, stir for 1 hour, filter, repeat the above steps twice, and combine the filtrates;

[0029] (3) Ethanol precipitation: Take the filtrate of step (2), add 2 times of 95% ethanol, stir well, let it stand overnight, siphon the upper alcohol solution, and dry it in vacuum to obtain heparin sodium solid;

[003...

Embodiment 2

[0035] A method for separating and purifying high-quality heparin sodium, comprising the steps of:

[0036] (1) Salt solution:

[0037] Take the aqueous solution of crude heparin sodium, add NaCl to make the NaCl content 4wt%, adjust the pH value to 9-10, salt it at 40°C for 2 hours, cool it down to 10°C, let stand, centrifuge, and collect the supernatant;

[0038] (2) Adsorption and elution:

[0039] Take the supernatant obtained in step (1) and the pretreated D-254 anion resin and stir for 2 hours at a stirring speed of 60r / min, filter and discard the filtrate; wash the anion resin with 3wt% NaCl aqueous solution; then use 12%wtNaCl aqueous solution Soak the anion resin, stir for 1 hour, filter, repeat the above steps twice, and combine the filtrates;

[0040] (3) Ethanol precipitation: Take the filtrate of step (2), add 2 times of 95% ethanol, stir well, let it stand overnight, siphon the upper alcohol solution, and dry it in vacuum to obtain heparin sodium solid;

[004...

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PUM

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Abstract

The invention discloses a separation purification method of refined heparin sodium. The method comprises the steps of: (1) salt hydrolysis; (2) adsorption and elution; (3) ethanol precipitation; (4) first oxidation; (5) secondary oxidation; and (6) precipitation. The method provided by the invention purifies crude heparin sodium by a two-step oxidation, not only reduces the production cost, and has no need for special equipment, also the prepared refined heparin sodium product has good purity and high recovery rate, and the quality and activity of heparin sodium are ensured.

Description

technical field [0001] The invention belongs to the field of chemical industry and relates to a method for separating and purifying high-quality heparin sodium. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, has anticoagulant effect, and is widely used in surgery, treatment of cerebral thrombosis, myocardial infarction, etc. At present, heparin sodium is mainly extracted from the small intestine mucosa of pigs and sheep, bovine lungs, salted sausage casings, etc. [0003] Heparin sodium widely exists in mammalian liver, lung, and intestinal mucosa, and mostly exists in complexes combined with proteins. Enzyme hydrolyzed protein can separate heparin sodium, which is mucopolysaccharide containing sulfuric acid, amino group and aldonic acid. At pH 8-9, it is negatively charged, and can perform ion exchange with anion exchangers for rough separation, and the polysaccharide solution is precipitated in high-concentration et...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 方蕊
Owner ANHUI IND & TRADE VOCATIONAL TECHN COLLEGE
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